1.1.1.274: 2,5-didehydrogluconate reductase (2-dehydro-D-gluconate-forming)
This is an abbreviated version!
For detailed information about 2,5-didehydrogluconate reductase (2-dehydro-D-gluconate-forming), go to the full flat file.
Word Map on EC 1.1.1.274
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1.1.1.274
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2,5-diketo-d-gluconic
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corynebacterium
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2-keto-l-gulonic
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nadph-dependent
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aldo-keto
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l-ascorbic
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d-glucose
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synthesis
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citrinum
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penicillium
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activity-stained
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aldose
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cofactor-binding
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reductases
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biocatalysis
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erwinia
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error-prone
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stereo-specific
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ascorbate
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enantioselectivity
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two-phase
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pantoea
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citrea
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biotechnology
- 1.1.1.274
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2,5-diketo-d-gluconic
- corynebacterium
-
2-keto-l-gulonic
-
nadph-dependent
-
aldo-keto
-
l-ascorbic
- d-glucose
- synthesis
- citrinum
-
penicillium
-
activity-stained
- aldose
-
cofactor-binding
- reductases
-
biocatalysis
- erwinia
-
error-prone
-
stereo-specific
- ascorbate
-
enantioselectivity
-
two-phase
-
pantoea
- citrea
- biotechnology
Reaction
Synonyms
2,5-diketo-D-gluconate reductase, 2,5-diketo-D-gluconic acid reductase, 2,5-diketo-gluconate reductase, 2,5-DKG, 2,5-DKG reductase, 2,5-DKGR, 2,5DKGR, beta-keto ester reductase, CTATCC11996_22452, Dkr, YafB, YqhE, YqhE reductase
ECTree
1.1.1.274 2,5-didehydrogluconate reductase (2-dehydro-D-gluconate-forming)Advanced search results
results (
results (Substrates Products
Substrates Products on EC 1.1.1.274 - 2,5-didehydrogluconate reductase (2-dehydro-D-gluconate-forming)
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2,5-didehydro-D-gluconate + NADPH + H+
2-dehydro-D-gluconate + NADP+
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-
-
?
ethyl 2-ethylacetoacetate + NADPH
ethyl (2R)-ethyl-(3S)-hydroxybutanoate + NADP+
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stereospecific reaction
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-
?
ethyl 2-methylacetoacetate + NADPH
ethyl (2R)-methyl-(3S)-hydroxybutanoate + NADP+
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stereospecific reaction
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-
?
ethyl acetoacetate + NADPH
ethyl (3S)-hydroxybutanoate + NADP+
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stereospecific reaction
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-
?
ethyl-(2R)-allylacetoacetate + NADPH
?
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250% of activity with ethyl-2-methylacetoacetate
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-
?
2,5-didehydro-D-gluconate + NADH
2-keto-L-gulonate + NAD+
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170fold lower reduction rate with NADH compared to NADPH
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?
2,5-didehydro-D-gluconate + NADH
2-keto-L-gulonate + NAD+
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170fold lower reduction rate with NADH compared to NADPH
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?
2,5-didehydro-D-gluconate + NADPH
2-keto-L-gulonate + NADP+
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-
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?
2,5-didehydro-D-gluconate + NADPH
2-keto-L-gulonate + NADP+
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stereospecific reduction to 2-keto-L-gulonate, no activity with D-fructose, L-sorbose, 5-keto-D-gluconate, 2-keto-L-gulonate, 2-keto-D-gluconate, pyruvate or hydroxypyruvate
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r
2,5-didehydro-D-gluconate + NADPH
2-keto-L-gulonate + NADP+
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stereospecific reduction to 2-keto-L-gulonate, no activity with D-fructose, L-sorbose, 5-keto-D-gluconate, 2-keto-L-gulonate, 2-keto-D-gluconate, pyruvate or hydroxypyruvate
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r
2,5-didehydro-D-gluconate + NADPH
2-keto-L-gulonate + NADP+
-
-
-
?
2,5-didehydro-D-gluconate + NADPH
2-keto-L-gulonate + NADP+
both isoenzymes A and B are specific for NADPH
-
?
2,5-didehydro-D-gluconate + NADPH
2-keto-L-gulonate + NADP+
-
-
-
?
2,5-didehydro-D-gluconate + NADPH
2-oxo-L-gulonic acid + NADP+
step in the biosynthesis of L-ascorbic acid
-
-
?
2,5-didehydro-D-gluconate + NADPH
2-oxo-L-gulonic acid + NADP+
i.e. 2,5-diketo-D-gluconic acid or 2,5-DKG, stereospecific reaction
i.e. 2-keto-L-gulonic acid or 2-KLG, product is a precursor for L-ascorbic acid
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?
2-dehydro-D-gulonate + NADP+
2,5-didehydro-D-gluconate + NADPH + H+
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-
-
-
?
2-dehydro-D-gulonate + NADP+
2,5-didehydro-D-gluconate + NADPH + H+
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-
-
-
?
5-keto-D-fructose + NADPH
L-sorbose + NADP+
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isoenzymes I and II
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?
5-keto-D-fructose + NADPH
L-sorbose + NADP+
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isoenzymes I and II
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?
ethyl 2-methylacetoacetate + NADPH + H+
ethyl (2R)-methyl-(3S)-hydroxybutanoate + NADP+
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7% activity with NADH
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?
ethyl 2-methylacetoacetate + NADPH + H+
ethyl (2R)-methyl-(3S)-hydroxybutanoate + NADP+
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7% activity with NADH
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?
ethyl-(2R)-ethylacetoacetate + NADPH
ethyl-(2R)-ethyl-(3S)-hydroxybutanoate + NADP+
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120% of activity with ethyl-2-methylacetoacetate
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?
ethyl-(2R)-ethylacetoacetate + NADPH
ethyl-(2R)-ethyl-(3S)-hydroxybutanoate + NADP+
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120% of activity with ethyl-2-methylacetoacetate
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?
ethylacetoacetate + NADPH
ethyl-(3S)-hydroxybutanoate + NADP+
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53% of activity with ethyl-2-methylacetoacetate
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?
ethylacetoacetate + NADPH
ethyl-(3S)-hydroxybutanoate + NADP+
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53% of activity with ethyl-2-methylacetoacetate
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?
additional information
?
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the enzyme catalyzes degradation of estradiol, estrone, testosterone, and methyltestosterone, overview
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?
additional information
?
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the enzyme catalyzes degradation of estradiol, estrone, testosterone, and methyltestosterone, overview
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?
additional information
?
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isozyme A is more stable than isozyme B but less active
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-
?
additional information
?
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substrate specificity, no activity with ethyl propionyl acetate and ethyl butyryl acetate
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-
?
additional information
?
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substrate specificity, no activity with ethyl propionyl acetate and ethyl butyryl acetate
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?
