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1.1.1.274: 2,5-didehydrogluconate reductase (2-dehydro-D-gluconate-forming)

This is an abbreviated version!
For detailed information about 2,5-didehydrogluconate reductase (2-dehydro-D-gluconate-forming), go to the full flat file.

Word Map on EC 1.1.1.274

Reaction

2-dehydro-D-gluconate
+
NADP+
=
2,5-didehydro-D-gluconate
+
NADPH
+
H+

Synonyms

2,5-diketo-D-gluconate reductase, 2,5-diketo-D-gluconic acid reductase, 2,5-diketo-gluconate reductase, 2,5-DKG, 2,5-DKG reductase, 2,5-DKGR, 2,5DKGR, beta-keto ester reductase, CTATCC11996_22452, Dkr, YafB, YqhE, YqhE reductase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.274 2,5-didehydrogluconate reductase (2-dehydro-D-gluconate-forming)

Purification

Purification on EC 1.1.1.274 - 2,5-didehydrogluconate reductase (2-dehydro-D-gluconate-forming)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, DEAE-Sepharose, Matrix Red-A, Sephacryl S-200, hypatite C
-
DEAE-cellulose, Cibacron blue F3GA agarose, gel filtration
-
DEAE-Sepharose column chromatography and Superdex 200 gel filtration
-
native enzyme from strain BL21(DE3), 730fold to homogeneity by ammonium sulfate fractionation, DEAE ion exchange and Red-A affinity chromatography, ultrafiltration, hypatite C-resin chromatography, and a second ultrafiltration step
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant isoenzyme A
-
recombinant isoenzyme A, Red A dye-affinity, anion exchange, gel filtration
recombinant isoenzymes A and B, affinity chitin-binding tag