1.1.1.274: 2,5-didehydrogluconate reductase (2-dehydro-D-gluconate-forming)
This is an abbreviated version!
For detailed information about 2,5-didehydrogluconate reductase (2-dehydro-D-gluconate-forming), go to the full flat file.
Word Map on EC 1.1.1.274
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1.1.1.274
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2,5-diketo-d-gluconic
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corynebacterium
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2-keto-l-gulonic
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nadph-dependent
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aldo-keto
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l-ascorbic
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d-glucose
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synthesis
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citrinum
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penicillium
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activity-stained
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aldose
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cofactor-binding
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reductases
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biocatalysis
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erwinia
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error-prone
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stereo-specific
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ascorbate
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enantioselectivity
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two-phase
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pantoea
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citrea
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biotechnology
- 1.1.1.274
-
2,5-diketo-d-gluconic
- corynebacterium
-
2-keto-l-gulonic
-
nadph-dependent
-
aldo-keto
-
l-ascorbic
- d-glucose
- synthesis
- citrinum
-
penicillium
-
activity-stained
- aldose
-
cofactor-binding
- reductases
-
biocatalysis
- erwinia
-
error-prone
-
stereo-specific
- ascorbate
-
enantioselectivity
-
two-phase
-
pantoea
- citrea
- biotechnology
Reaction
Synonyms
2,5-diketo-D-gluconate reductase, 2,5-diketo-D-gluconic acid reductase, 2,5-diketo-gluconate reductase, 2,5-DKG, 2,5-DKG reductase, 2,5-DKGR, 2,5DKGR, beta-keto ester reductase, CTATCC11996_22452, Dkr, YafB, YqhE, YqhE reductase
ECTree
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Crystallization
Crystallization on EC 1.1.1.274 - 2,5-didehydrogluconate reductase (2-dehydro-D-gluconate-forming)
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10 mg/ml purified recombinant F22Y/K232G/R238H/A272G mutant enzyme in complex with NADH in 25 mM Tris-HCl, pH 7.5, 1 mM NADH, hanging drop vapour diffusion method, room temperature, equal volumes, 0.005 ml each, of protein and crystallization solution against 1 ml reservoir crystallization solution containing 1.5 M lithium sulfate, 0.1 M Na-HEPES, pH 7.5, 7-10 days, X-ray diffraction structure determination and analysis at 2.0 A resolution, molecular replacment, molecular modeling of substrate and cofactor binding
isoenzyme A, hanging drop-vapour diffusion, 1.9 A resolution of apo enzyme
isoenzyme A, hanging-drop vapour diffusion, X-ray structure of complex with NADPH, 2.1 A resolution
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