VvSDH isozymes expression is analysed in the main berry tissues (skin, pulp, and seeds) at three development stages (green stage, veraison, and maturity) to determine their spatial and temporal expression patterns, overview
determination of the variability in flowering phenology of Scots pine (Pinus sylvestris L.) clones in a seed orchard, genetic structure and genetic markers (13 isozyme loci and 5 chloroplast and 3 nuclear DNA microsatellite loci) among groups of clones that are differentisated by flowering phenology, overview. The frequency of allele 2 at the shikimate dehydrogenase A locus (ShDH A 2) differs significantly between the groups of early- and late-flowering trees and between the groups of intermediate- and late-flowering trees. In addition, a significant difference in the frequencies of the genotype ShDH A 11 is observed between the intermediate- and late-flowering groups, the ShDH A locus might be considered as isoenzymatic marker that differentiates these flowering groups of Scots pine clones
Escherichia coli strain PB12.SA22 and the derivatives ydiB- and ydiB+ are evaluated for their ability to produce shikimate (SA), quinate (QA), 3-dehydroshikimate (DHS), and 3-dehydroquinate (DHQ) in batch culture fermentations growing in 1-l fermentors using 500 ml of a mineral broth supplemented with 25 g/l glucose and 15 g/l YE. Biomass and glucose consumption and the production of aromatic intermediates of the SA pathway, SA, QA, DHQ, and DHS are determined for all derivatives, overview. The highest production of DHQ and DHS is 0.07 and 0.074 g/l, respectively. SA and QA are produced during the early exponential stage, as these compounds are detected during the first 5 h of cultivation (SA = 0.49 g/l and QA = 0.38 g/l, respectively). In the stationary stage and until 20 h of cultivation, this strain consumes the remaining residual glucose. From this time until the end of fermentation, the supernatant concentration of detected SA shows no significant changes, reaching 8.2 g/l by the end of fermentation (50 h), whereas the final QA concentration is 1.52 g/l
Escherichia coli strain PB12.SA22 and the derivatives ydiB- and ydiB+ are evaluated for their ability to produce shikimate (SA), quinate (QA), 3-dehydroshikimate (DHS), and 3-dehydroquinate (DHQ) in batch culture fermentations growing in 1-l fermentors using 500 ml of a mineral broth supplemented with 25 g/l glucose and 15 g/l YE. Biomass and glucose consumption and the production of aromatic intermediates of the SA pathway, SA, QA, DHQ, and DHS are determined for all derivatives, overview. The highest production of DHQ and DHS is 0.07 and 0.074 g/l, respectively. SA and QA are produced during the early exponential stage, as these compounds are detected during the first 5 h of cultivation (SA = 0.49 g/l and QA = 0.38 g/l, respectively). In the stationary stage and until 20 h of cultivation, this strain consumes the remaining residual glucose. From this time until the end of fermentation, the supernatant concentration of detected SA shows no significant changes, reaching 8.2 g/l by the end of fermentation (50 h), whereas the final QA concentration is 1.52 g/l
comparison of EcDQD/SDH, UGT84A25a/b, and UGT84A26a/b expression patterns in Eucalyptus camaldulensis, relative EcDQD/SDH mRNA levels in the leaves, stems, and roots are plotted against the relative UGT84A25a/b and UGT84A26a/b mRNA levels in the same samples, and determination of concentrations of the metabolites in the different tissues, overview
comparison of EcDQD/SDH, UGT84A25a/b, and UGT84A26a/b expression patterns in Eucalyptus camaldulensis, relative EcDQD/SDH mRNA levels in the leaves, stems, and roots are plotted against the relative UGT84A25a/b and UGT84A26a/b mRNA levels in the same samples, and determination of concentrations of the metabolites in the different tissues, overview
comparison of EcDQD/SDH, UGT84A25a/b, and UGT84A26a/b expression patterns in Eucalyptus camaldulensis, relative EcDQD/SDH mRNA levels in the leaves, stems, and roots are plotted against the relative UGT84A25a/b and UGT84A26a/b mRNA levels in the same samples, and determination of concentrations of the metabolites in the different tissues, overview
comparison of EcDQD/SDH, UGT84A25a/b, and UGT84A26a/b expression patterns in Eucalyptus camaldulensis, relative EcDQD/SDH mRNA levels in the leaves, stems, and roots are plotted against the relative UGT84A25a/b and UGT84A26a/b mRNA levels in the same samples, and determination of concentrations of the metabolites in the different tissues, overview