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1.1.1.25: shikimate dehydrogenase (NADP+)

This is an abbreviated version!
For detailed information about shikimate dehydrogenase (NADP+), go to the full flat file.

Word Map on EC 1.1.1.25

Reaction

shikimate
+
NADP+
=
3-dehydroshikimate
 +
NADPH
 +
H+

Synonyms

3-dehydroquinate dehydratase/shikimate dehydrogenase, 5-dehydroshikimate reductase, 5-dehydroshikimic reductase, ael1, Af2327, AroE, bifunctional dehydroquinate dehydratase-shikimate dehydrogenase, bifunctional DHQD-SDH/QDH, cgR_0495, cgR_1677, ChlreSDH, CsDQD/SDH, dehydroquinate dehydratase-shikimate dehydrogenase, dehydroquinate dehydratase/shikimate dehydrogenase, dehydroshikimic reductase, DHD/SHD, DHQ-SDH, DHQD-SDH, DHS reductase, DQD/SDH, EcDQD, EcDQD/SDH, EcDQD/SDH1, EcDQD/SDH2, EcDQD/SDH3, EcoSDH, EcSDH, EMB3004, HI0607, HpSDH, More, MtbSD, MtbSDH, NAD+-dependent enzyme quinate/shikimate dehydrogenase, NADP-dependent shikimate dehydrogenase, NADP-shikimate dehydrogenase, PgSDH, PhypaSDH, PintaSDH, Poptr1, Poptr5, PoptrSDH1, QDH/SDH, qsuD, quinate/shikimate dehydrogenase, RhobaSDH, rifI, SaSDH, SD, SDH, sdhL, SelmoSDH, ShDH, shikimate 5-dehydrogenase, shikimate dehydrogenase, shikimate oxidoreductase, shikimate:NADP oxidoreductase, shikimate:NADP+ 5-oxidoreductase, shikimate:NADP+ oxidoreductase, SKDH, TgSDH, tm0346, VvSDH1, VvSDH2, VvSDH3, VvSDH4, YdiB

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.25 shikimate dehydrogenase (NADP+)

Cloned

Cloned on EC 1.1.1.25 - shikimate dehydrogenase (NADP+)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis
DNA and amino acid sequence determination and analysis, expression of Hs6-tagged isozyme 1 in Escherichia coli, transfection of Nicotina plants
expression analysis, phylogenetic analysis
expression in Escherichia coli
expression in Escherichia coli as recombinant N-terminal GST-tagged protein
expression in Escherichia coli in native and His-tagged form
expression of C-terminally His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
-
expression of wild-type and selenomethionine-labeled enzyme in Escherichia coli strain DL41
five putative SDH-encoding genes amplified and cloned into either a modified pET28a vector or the p15TVLIC vector, a ligation-independent vector due to incompatible cloning sites between the insert and pET28.3. Expressed in either the Escherichia coli strain BL21 gold (p15TV-LIC constructs) or the Escherichia coli BL21CodonPlus (pET28.3constructs)
gene aroE from Escherichia coli sstrain DH5alpha, recombinant overexpression in Saccharomyces cerevisiae strain CEN.PK 113-5D yielding strain CEN.PK-aroE
gene aroE, DNA and amino acid sequence determination, functional expression as soluble enzyme in Escherichia coli strain BL21(DE3)
gene aroE, expression of GST-tagged SeMet-labeled enzyme in Escherichia coli strain BL21
gene aroE, expression of His-tagged AroE in Escherichia coli strain BL21(DE3)
-
gene aroE, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene aroE, overexpression in the aroE auxotrophic mutant strain AB2834
-
gene aroE, recombinant expression
gene aroE, recombinant expression in Escherichia coli strain JM101
gene aroE, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli
gene aroE, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21 CodonPlus
gene aroE, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS
-
gene EcDQD/SDH1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21-CodonPlus(DE3)-RIL, quantitative real-time RT-PCR enzyme expression analysis
gene EcDQD/SDH2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21-CodonPlus(DE3)-RIL, quantitative real-time RT-PCR enzyme expression analysis
gene EcDQD/SDH3, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21-CodonPlus(DE3)-RIL, quantitative real-time RT-PCR enzyme expression analysis
gene loci ShDH A and ShDH B, genetic variability, overview
-
gene SDH, phylogenetic analysis
gene SDH1, phylogenetic analysis
-
gene ydiB, the ydiB gene is cloned into plasmid pTOPO aroB aroE, resulting in the pTOPO ydiB aroB aroE derivative, enzyme overexpression in Escherichia coli strain PB12, quantitative RT-PCR analysis, coexpression of plasmid pTOPO aroB aroE and pJLB aroG fbr tktA and the cultivation of this derivative in Escherichia coli strain PB12.SA2 resulting in very high level expression of gene ydiB during exponential and stationary growth stages
genes encoding PgSDH1, PgSDH3-1,2, PgSDH3a-1,2, and PgSDH4, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, genes PgSDH3 and PgSDH3a occur in tandem
isozyme VvSDH1, sequence comparison and phylogenetic analysis and tree, recombinant expression
isozyme VvSDH2, sequence comparison and phylogenetic analysis and tree, recombinant expression
isozyme VvSDH3, sequence comparison and phylogenetic analysis and tree, recombinant expression
isozyme VvSDH4, sequence comparison and phylogenetic analysis and tree, recombinant expression
multiple DQD/SDH protein sequences are obtained from Phytozome 12.0 and NCBI, phylogenetic analysis and tree, recombinant expression of CsDQD/SDH isozymes in Escherichia coli strain NovaBlue (DE3)
overexpression in Escherichia coli
overexpression in Escherichia coli K12
-
overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
phylogenetic analysis of the three paralogues YdiB, AroE, and HI0607, DNA and amino acid sequence determination and analysis of paralogue HI0607, expression of wild-type paralogue HI0607 in Escherichia coli strain BL21(DE3) and of selenomethionine-labeled enzyme in strain B834(DE3)
-
plasmid pET23a(+)::aroE (either wild-type or mutants) transformed into Escherichia coli C41(DE3)
-
recombinant His6-tagged DQD/SDH isozyme 1 overexpression in Escherichia coli strain M15
-
recombinant His6-tagged DQD/SDH isozyme 5 overexpression in Escherichia coli strain M15
-
SDH-encoding gene is amplified by polymerase chain reaction from Pseudomonas putida KT2440 genomic DNA using primers with incorporated restriction sites. Resulting product is cloned into a vector and expressed in the Escherichia coli strain BL21 with appropriate antibiotics.
SDH-encoding gene is amplified by polymerase chain reaction from Pseudomonas putida KT2440 genomic DNA using primers with incorporated restriction sites. Resulting product is cloned into vector and expressed in the Escherichia coli strain BL21 with appropriate antibiotics.
wild-type and mutant cloned into vector pET22b and transformed into Escherichia coli strain BL21(DE3). Selenomethionine-substituted SDH generated in the methionine auxotrophic Escherichia coli strain B834(DE3)