1.1.1.238: 12beta-hydroxysteroid dehydrogenase

This is an abbreviated version!
For detailed information about 12beta-hydroxysteroid dehydrogenase, go to the full flat file.

Reaction

3alpha,7alpha,12beta-trihydroxy-5beta-cholan-24-oate

3alpha,7alpha-dihydroxy-12-oxo-5beta-cholan-24-oate

Synonyms

12beta-hydroxy steroid (nicotinamide adenine dinucleotide phosphate) dehydrogenase

ECTree

1 Oxidoreductases

1.1 Acting on the CH-OH group of donors

1.1.1 With NAD⁺ or NADP⁺ as acceptor

1.1.1.238 12beta-hydroxysteroid dehydrogenase

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Results	in table
1	Application
1	CAS Registry Number
2	Cofactor
1	General Stability
9	KM Value [mM]
1	Localization
1	Molecular Weight [Da]
1	Organism
2	Pathway
2	pH Optimum
1	pH Range
1	pH Stability
1	Reaction
3	Reaction Type
1	Reference
2	Specific Activity [micromol/min/mg]
1	Storage Stability
8	Substrates and Products (Substrate)
1	Synonyms
1	Systematic Name
3	Temperature Stability [°C]

KM Value

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KM Value on EC 1.1.1.238 - 12beta-hydroxysteroid dehydrogenase

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0.0895

3,12-Dioxo-5beta-cholanoate

Clostridium paraputrificum

286353

0.171

3,7,12-Trioxo-5beta-cholanoate

Clostridium paraputrificum

286353

0.142

3,7,12-trioxotaureate

Clostridium paraputrificum

286353

0.274

3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoate

Clostridium paraputrificum

286353

0.141

3alpha-Hydroxy-12-oxo-5beta-cholanoate

Clostridium paraputrificum

286353

0.144

3alpha-Hydroxy-7,12-dioxo-5beta-cholanoate

Clostridium paraputrificum

286353

0.0837

7,12-Dioxo-5beta-cholanoate

Clostridium paraputrificum

286353

0.138

7alpha-Hydroxy-3,12-dioxo-5beta-cholanoate

Clostridium paraputrificum

286353

0.0854

NADPH

Clostridium paraputrificum

286353