1.1.1.214: 2-dehydropantolactone reductase (Si-specific)
This is an abbreviated version!
For detailed information about 2-dehydropantolactone reductase (Si-specific), go to the full flat file.
Reaction
Synonyms
2-dehydropantoyl-lactone reductase (B-specific), 2-ketopantoyl lactone reductase, 2-oxopantoyl lactone reductase, conjugated polyketone reductase, conjugated polyketone reductase C2, CORT_0G03830, CPR, CPR-C1, CPR-C2, ketopantoyl lactone reductase, NADPH-dependent conjugated polyketone reductase, NADPH-dependent ketopantoyl lactone reductase, nicotinamide adenine dinucleotide phosphate-dependent ketopantoyl lactone reductase
ECTree
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Purification
Purification on EC 1.1.1.214 - 2-dehydropantolactone reductase (Si-specific)
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form A and form B of the enzyme obtained separated by rupture, centrifugation and column chromatography on DEAE-cellulose and hydroxylapatite
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recombinant His-tagged enzyme from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography, and tag cleavage by thrombin, followed by anion exchange chromatography, gel filtration, and dialysis
using affinity chromatography on Red-Agarose and several subsequent ion exchange steps
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