1.1.1.201: 7beta-hydroxysteroid dehydrogenase (NADP+)
This is an abbreviated version!
For detailed information about 7beta-hydroxysteroid dehydrogenase (NADP+), go to the full flat file.
Word Map on EC 1.1.1.201
-
1.1.1.201
-
synthesis
-
ursodeoxycholic
-
taurinates
-
dehydrogenases
-
nadph-dependent
-
aerofaciens
-
bile
- 1.1.1.201
- synthesis
-
ursodeoxycholic
-
taurinates
- dehydrogenases
-
nadph-dependent
- aerofaciens
- bile
Reaction
Synonyms
7beta-HSD, 7beta-HSDH, 7beta-hydroxysteroid dehydrogenase, More, NADP-dependent 7beta-hydroxysteroid dehydrogenase, RUMGNA_02585
ECTree
1.1.1.201 7beta-hydroxysteroid dehydrogenase (NADP+)Advanced search results
results (
results (Substrates Products
Substrates Products on EC 1.1.1.201 - 7beta-hydroxysteroid dehydrogenase (NADP+)
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
top
SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
3,7,12-triketo-5beta-cholanoic acid + NADPH
7beta-hydroxy-3,12-diketo-5beta-cholanoic acid + NADP+
-
-
-
?
3,7,12-triketo-5beta-cholanoyl taurine + NADPH
7beta-hydroxy-3,12-diketo-5beta-cholanoyl taurine + NADP+
-
-
-
?
3alpha,12alpha-dihydroxy-7-keto-5beta-cholanoic acid + NADPH
3alpha,7beta,12alpha-trihydroxy-5beta-cholanoic acid + NADP+
3alpha,7beta,12alpha-trihydroxy-5beta-cholanoic acid + NADP+
3alpha,12alpha-dihydroxy-7-keto-5beta-cholanoic acid + NADPH
3alpha,7beta-dihydroxy-5beta-cholan-24-oic acid + NADP+
7-oxo-lithocholic acid + NADPH + H+
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
3alpha-hydroxy-7-oxo-5beta-cholanoic acid + NADPH
3alpha,7beta-dihydroxy-5beta-cholanoyl glycine + NADP+
3alpha-hydroxy-7-keto-5beta-cholanoyl glycine + NADPH
3alpha,7beta-dihydroxy-5beta-cholanoyl taurine + NADP+
3alpha-hydroxy-7-keto-5beta-cholanoyl taurine + NADPH
3alpha-hydroxy-7,12-diketo-5beta-cholanoic acid + NADPH
3alpha,7beta-dihydroxy-12-keto-5beta-cholanoic acid + NADP+
3alpha-hydroxy-7-oxo-5beta-cholan-24-oic acid + NADPH + H+
3alpha,7beta-dihydroxy-5beta-cholan-24-oic acid + NADP+
5-cholesten-3beta,7beta-diol + NADP+
5-cholesten-3beta-ol-7-one + NADPH
-
i.e. 7beta-hydroxycholesterol
-
-
?
7,12-diketo-5beta-cholanoic acid + NADPH
7beta-hydroxy-12-keto-5beta-cholanoic acid + NADP+
corticosterone + NADP+
21-hydroxy-pregn-4-ene-3,11,20-trione + NADPH
-
oxidation at C-11
-
-
?
3,7-diketo-5beta-cholanoic acid + NADPH
7beta-hydroxy-3-keto-5beta-cholanoic acid + NADP+
-
-
-
?
3,7-diketo-5beta-cholanoic acid + NADPH
7beta-hydroxy-3-keto-5beta-cholanoic acid + NADP+
-
-
-
?
3alpha,12alpha-dihydroxy-7-keto-5beta-cholanoic acid + NADPH
3alpha,7beta,12alpha-trihydroxy-5beta-cholanoic acid + NADP+
-
-
-
?
3alpha,12alpha-dihydroxy-7-keto-5beta-cholanoic acid + NADPH
3alpha,7beta,12alpha-trihydroxy-5beta-cholanoic acid + NADP+
-
-
-
?
3alpha,7beta,12alpha-trihydroxy-5beta-cholanoic acid + NADP+
3alpha,12alpha-dihydroxy-7-keto-5beta-cholanoic acid + NADPH
-
partially reversible, poor substrate
-
r
3alpha,7beta,12alpha-trihydroxy-5beta-cholanoic acid + NADP+
3alpha,12alpha-dihydroxy-7-keto-5beta-cholanoic acid + NADPH
-
ursocholic acid
-
r
3alpha,7beta,12alpha-trihydroxy-5beta-cholanoic acid + NADP+
3alpha,12alpha-dihydroxy-7-keto-5beta-cholanoic acid + NADPH
-
ursocholic acid
-
?
3alpha,7beta-dihydroxy-5beta-cholan-24-oic acid + NADP+
7-oxo-lithocholic acid + NADPH + H+
i.e. ursodeoxycholic acid, exclusive specificity for the hydroxyl group in beta configuration at the C-7 position
-
-
r
3alpha,7beta-dihydroxy-5beta-cholan-24-oic acid + NADP+
7-oxo-lithocholic acid + NADPH + H+
i.e. ursodeoxycholic acid, exclusive specificity for the hydroxyl group in beta configuration at the C-7 position
-
-
r
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
3alpha-hydroxy-7-oxo-5beta-cholanoic acid + NADPH
-
ursodeoxycholic acid
-
?
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
3alpha-hydroxy-7-oxo-5beta-cholanoic acid + NADPH
-
ursodeoxycholic acid
-
?
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
3alpha-hydroxy-7-oxo-5beta-cholanoic acid + NADPH
-
only active on free and conjugated bile salts
-
?
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
3alpha-hydroxy-7-oxo-5beta-cholanoic acid + NADPH
-
ursodeoxycholic acid
-
?
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
3alpha-hydroxy-7-oxo-5beta-cholanoic acid + NADPH
-
ursodeoxycholic acid
-
?
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
3alpha-hydroxy-7-oxo-5beta-cholanoic acid + NADPH
-
activity only in the reductive reaction
-
-
ir
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
3alpha-hydroxy-7-oxo-5beta-cholanoic acid + NADPH
-
activity only in the reductive reaction
-
-
ir
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
3alpha-hydroxy-7-oxo-5beta-cholanoic acid + NADPH
-
ursodeoxycholic acid
-
?
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
3alpha-hydroxy-7-oxo-5beta-cholanoic acid + NADPH
-
ursodeoxycholic acid
-
r
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
3alpha-hydroxy-7-oxo-5beta-cholanoic acid + NADPH
-
partially reversible
-
r
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
3alpha-hydroxy-7-oxo-5beta-cholanoic acid + NADPH
-
only active on free and conjugated bile salts
-
?
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
3alpha-hydroxy-7-oxo-5beta-cholanoic acid + NADPH
-
ursodeoxycholic acid
-
?
3alpha,7beta-dihydroxy-5beta-cholanoic acid + NADP+
3alpha-hydroxy-7-oxo-5beta-cholanoic acid + NADPH
-
active on both free and conjugated bile salts
-
?
3alpha,7beta-dihydroxy-5beta-cholanoyl glycine + NADP+
3alpha-hydroxy-7-keto-5beta-cholanoyl glycine + NADPH
-
-
-
?
3alpha,7beta-dihydroxy-5beta-cholanoyl glycine + NADP+
3alpha-hydroxy-7-keto-5beta-cholanoyl glycine + NADPH
-
-
-
?
3alpha,7beta-dihydroxy-5beta-cholanoyl taurine + NADP+
3alpha-hydroxy-7-keto-5beta-cholanoyl taurine + NADPH
-
-
-
?
3alpha,7beta-dihydroxy-5beta-cholanoyl taurine + NADP+
3alpha-hydroxy-7-keto-5beta-cholanoyl taurine + NADPH
-
-
-
?
3alpha,7beta-dihydroxy-5beta-cholanoyl taurine + NADP+
3alpha-hydroxy-7-keto-5beta-cholanoyl taurine + NADPH
-
partially reversible, poore substrate
-
r
3alpha-hydroxy-7,12-diketo-5beta-cholanoic acid + NADPH
3alpha,7beta-dihydroxy-12-keto-5beta-cholanoic acid + NADP+
-
-
-
?
3alpha-hydroxy-7,12-diketo-5beta-cholanoic acid + NADPH
3alpha,7beta-dihydroxy-12-keto-5beta-cholanoic acid + NADP+
-
-
-
?
3alpha-hydroxy-7-oxo-5beta-cholan-24-oic acid + NADPH + H+
3alpha,7beta-dihydroxy-5beta-cholan-24-oic acid + NADP+
i.e. 7-oxo-lithocholic acid
i.e. ursodeoxycholic acid
-
r
3alpha-hydroxy-7-oxo-5beta-cholan-24-oic acid + NADPH + H+
3alpha,7beta-dihydroxy-5beta-cholan-24-oic acid + NADP+
i.e 7-oxo-lithocholic acid, the forward reaction is preferred, formation of ursodeoxycholic acid
i.e. ursodeoxycholic acid
-
r
3alpha-hydroxy-7-oxo-5beta-cholan-24-oic acid + NADPH + H+
3alpha,7beta-dihydroxy-5beta-cholan-24-oic acid + NADP+
i.e. 7-oxo-lithocholic acid
i.e. ursodeoxycholic acid
-
r
3alpha-hydroxy-7-oxo-5beta-cholan-24-oic acid + NADPH + H+
3alpha,7beta-dihydroxy-5beta-cholan-24-oic acid + NADP+
i.e 7-oxo-lithocholic acid, the forward reaction is preferred, formation of ursodeoxycholic acid
i.e. ursodeoxycholic acid
-
r
7,12-diketo-5beta-cholanoic acid + NADPH
7beta-hydroxy-12-keto-5beta-cholanoic acid + NADP+
-
-
-
?
7,12-diketo-5beta-cholanoic acid + NADPH
7beta-hydroxy-12-keto-5beta-cholanoic acid + NADP+
-
-
-
?
7,12-dioxo-ursodeoxycholic acid + NADPH + H+
12-oxo-ursodeoxycholic acid + NADP+
-
-
-
-
?
7,12-dioxo-ursodeoxycholic acid + NADPH + H+
12-oxo-ursodeoxycholic acid + NADP+
-
-
-
-
?
7,12-dioxo-ursodeoxycholic acid + NADPH + H+
12-oxo-ursodeoxycholic acid + NADP+
-
-
-
-
?
7,12-dioxo-ursodeoxycholic acid + NADPH + H+
12-oxo-ursodeoxycholic acid + NADP+
-
-
-
-
?
7,12-dioxo-ursodeoxycholic acid + NADPH + H+
12-oxo-ursodeoxycholic acid + NADP+
-
-
-
-
?
7-ketolithocholic acid + NADPH + H+
ursodeoxycholic acid + NADP+
-
-
only product, 90% conversion, 71% yield
-
r
7-ketolithocholic acid + NADPH + H+
ursodeoxycholic acid + NADP+
-
-
only product, 90% conversion, 71% yield
-
r
7-oxo-lithocholic acid + NADPH + H+
ursodeoxycholic acid + NADP+
-
-
-
-
?
7-oxo-lithocholic acid + NADPH + H+
ursodeoxycholic acid + NADP+
-
after a first reaction step with EC 1.1.1.159, 7alpha-hydroxysteroid dehydrogenase, from chenodeoxycholic acid
-
-
?
7-oxo-lithocholic acid + NADPH + H+
ursodeoxycholic acid + NADP+
[Ruminococcus] torques ATCC 35915
-
after a first reaction step with EC 1.1.1.159, 7alpha-hydroxysteroid dehydrogenase, from chenodeoxycholic acid
-
-
?
7-oxo-lithocholic acid + NADPH + H+
ursodeoxycholic acid + NADP+
[Ruminococcus] torques ATCC 35915
-
-
-
-
?
7beta-hydroxysteroid + NADP+
7-oxosteroid + NADPH + H+
-
-
-
r
7beta-hydroxysteroid + NADP+
7-oxosteroid + NADPH + H+
stereoselective with 7-beta-hydroxysteroids
-
-
r
7beta-hydroxysteroid + NADP+
7-oxosteroid + NADPH + H+
-
-
-
r
7beta-hydroxysteroid + NADP+
7-oxosteroid + NADPH + H+
stereoselective with 7-beta-hydroxysteroids
-
-
r
chenodeoxycholic acid + NADPH + H+
ursodeoxycholic acid + NADP+
-
-
-
-
?
chenodeoxycholic acid + NADPH + H+
ursodeoxycholic acid + NADP+
-
-
-
-
?
chenodeoxycholic acid + NADPH + H+
ursodeoxycholic acid + NADP+
-
-
-
-
?
chenodeoxycholic acid + NADPH + H+
ursodeoxycholic acid + NADP+
-
-
-
-
?
dehydrocholic acid + NADPH + H+
12-oxo-ursodeoxycholic acid + NADP+
-
-
-
-
?
dehydrocholic acid + NADPH + H+
12-oxo-ursodeoxycholic acid + NADP+
-
-
-
-
?
dehydrocholic acid + NADPH + H+
12-oxo-ursodeoxycholic acid + NADP+
-
-
-
-
?
dehydrocholic acid + NADPH + H+
12-oxo-ursodeoxycholic acid + NADP+
-
-
-
-
?
dehydrocholic acid + NADPH + H+
12-oxo-ursodeoxycholic acid + NADP+
-
-
-
-
?
lithocholic acid + NADPH + H+
ursodeoxycholic acid + NADP+
-
presence of both 7alpha-HSDH (EC 1.1.1.159) and 7beta-HSDH in one organism allows epimerization by a single bacterium
-
-
?
lithocholic acid + NADPH + H+
ursodeoxycholic acid + NADP+
-
presence of both 7alpha-HSDH (EC 1.1.1.159) and 7beta-HSDH in one organism allows epimerization by a single bacterium
-
-
?
lithocholic acid + NADPH + H+
ursodeoxycholic acid + NADP+
-
presence of both 7alpha-HSDH (EC 1.1.1.159) and 7beta-HSDH in one organism allows epimerization by a single bacterium
-
-
?
lithocholic acid + NADPH + H+
ursodeoxycholic acid + NADP+
-
-
-
-
?
lithocholic acid + NADPH + H+
ursodeoxycholic acid + NADP+
-
presence of both 7alpha-HSDH (EC 1.1.1.159) and 7beta-HSDH in one organism allows epimerization by a single bacterium
-
-
?
lithocholic acid + NADPH + H+
ursodeoxycholic acid + NADP+
-
presence of both 7alpha-HSDH (EC 1.1.1.159) and 7beta-HSDH in one organism allows epimerization by a single bacterium
-
-
?
ursodeoxycholic acid + NADP+
7-ketolithocholic acid + NADPH + H+
-
-
-
-
r
ursodeoxycholic acid + NADP+
7-ketolithocholic acid + NADPH + H+
-
-
-
-
r
ursodeoxycholic acid + NADP+
7-oxo-lithocholic acid + NADPH + H+
-
-
-
r
ursodeoxycholic acid + NADP+
7-oxo-lithocholic acid + NADPH + H+
-
-
-
r
additional information
?
-
no activity with chenodeoxycholic acid, i.e. 3alpha,7alpha-dihydroxy-5beta-cholan-24-oic acid
-
-
?
additional information
?
-
no activity with chenodeoxycholic acid, i.e. 3alpha,7alpha-dihydroxy-5beta-cholan-24-oic acid
-
-
?
additional information
?
-
-
enzyme might also show 11beta-hydroxysteroid dehydrogenase activity, no activity with cholesterol, 7alpha-hydroxycholesterol, 25-hydroxycholesterol, pregnenolone, dehydroepiandrosterone, chenodoxycolic acid, and ursodeoxycholic acid
-
-
?
additional information
?
-
in vivo, the forward reaction that produces ursodeoxycholic acid from 7-oxo-lithocholic acid is observed to have a growth-dependent conversion rate of 90-100% after culture in anaerobic medium broth containing 1 mM 7-oxo-lithocholic acid, while the reverse reaction is undetectable. The purified 7beta-HSDH shows a kcat/Km value that is about 55fold higher for the forward reaction than for the reverse reaction, indicating that the enzyme favors the UDCA producing reaction
-
-
?
additional information
?
-
in vivo, the forward reaction that produces ursodeoxycholic acid from 7-oxo-lithocholic acid is observed to have a growth-dependent conversion rate of 90-100% after culture in anaerobic medium broth containing 1 mM 7-oxo-lithocholic acid, while the reverse reaction is undetectable. The purified 7beta-HSDH shows a kcat/Km value that is about 55fold higher for the forward reaction than for the reverse reaction, indicating that the enzyme favors the UDCA producing reaction
-
-
?
