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1.1.1.2: alcohol dehydrogenase (NADP+)

This is an abbreviated version!
For detailed information about alcohol dehydrogenase (NADP+), go to the full flat file.

Word Map on EC 1.1.1.2

Reaction

a primary alcohol
+
NADP+
=
an aldehyde
+
NADPH
+
H+

Synonyms

2° Adh, 3-DG-reducing enzyme, A3L14_07690, AAur_2040, ADH, ADH-1, ADH-2, ADH-I, ADH-II, ADH2, Adh319, ADH4, Adh6, AdhA, AdhB, AdhD, AKR1A1, AKR1A4, alcohol dehydrogenase C, alcohol dehydrogenase D, alcohol dehydrogenase [NADP(+)], Alcohol dehydrogenase [NADP+], aldehyde reductase, aldehyde reductase (NADPH2), aldehyde/ketone reductase, aldo-keto reductase, Aldo-keto reductase family 1 member A1, aldose reductase, ALDR, alipathic aldehyde reductase, ALR, ALR 1, ALR1, BdhA, bovine brain aldehyde reductase, CbADH, D-glucuronate reductase, daunorubicin reductase, DRD, EhADH1, FeADH, Gre2p, hexogenate dehydrogenase, high-Km aldehyde reductase, HvADH2, iron-containing alcohol dehydrogenase, KpADH, L-hexonate dehydrogenase, LB-RADH, LBADH, liver alcohol dehydrogenase, low-Km aldehyde reductase, mevaldate reductase, Mpd1, NADP(H)-dependent alcohol dehydrogenase, NADP+-dependent alcohol dehydrogenase, NADP-alcohol dehydrogenase, NADP-aldehyde reductase, NADP-dependent aldehyde reductase, NADP-linked aryl alcohol dehydrogenase, NADPH-aldehyde reductase, NADPH-cytochrome c reductase, NADPH-dependent ADH, NADPH-dependent aldehyde reductase, NADPH-dependent FALDR, NADPH-dependent fatty aldehyde reductase, NADPH-linked aldehyde reductase, nonspecific succinic semialdehyde reductase, Octopine dehydrogenase, putative iron alcohol dehydrogenase, PyAeADHII, rabbit kidney aldehyde-ketone reductase, RADH, ScADHVI, short-chain ADH, short-chain alcohol dehydrogenase, short-chain dehydrogenase/reductase, TBADH, TbADH1, Teth39_1597, Teth514_0564, TPN-L-hexonate dehydrogenase, TPNH-linked aldehyde reductase, TPNH-specific aldehyde reductase, triphosphopyridine nucleotide-linked aldehyde reductase, TsAdh319, Y63 protein, yeast alcohol dehydrogenase, YqhD

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.2 alcohol dehydrogenase (NADP+)

Cloned

Cloned on EC 1.1.1.2 - alcohol dehydrogenase (NADP+)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
1° Adh cloned and characterized
ADH gene cloned and overexpressed in Escherichia coli
-
alrA gene PCR amplified, cloned and highly expressed in Escherichia coli JM109
-
cloned into the pkk177-3H vector, transformation in Escherichia coli
-
Escherichia coli BL21, LB-medium, supplied with kanamycin (0,05 mg/L final concentration)
-
expressed in Escherichia coli
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli BL21 Star, 27°C, Lb-medium, carbenicillin, kanamycin, plasmid pBtacLB-ADH + pBBR1MCS2fdh, 130rpm
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli M15, plasmid pQE30-alr0307, cultivation in LB medium, ampicillin, kanamycin, 37°C
-
expressed in Escherichia coli strain TB1 as a fusion protein with the maltose binding protein on the N-terminus
Marinobacter nauticus VT8
-
expression in Escherichia coli
expression in Escherichia coli M15, plasmid pQE30-ALR
-
expression in Escherichia coli, wild-type and mutant enzyme
expression in Saccharomyces cerevisiae
expression in vector pET28a
-
expression of wild-type and mutant enzymes
-
frog stomach ADH cDNA cloned
-
gene A3L14_07690, recombinant enzyme expression in Escherichia coli strain Rosetta2
gene cloned and sequenced
-
gene coding for TBADH cloned and overexpressed in Escherichia coli
-
gene encoding ADHC cloned and sequenced
-
gene encoding NADP-dependent alcohol dehydrogenase cloned and sequenced
-
gene encoding NADPH group III alcohol dehydrogenase cloned and overexpressed in Escherichia coli
-
into the pET-28a(+) vector, plasmid pETAdhA expressed in Escherichia coli BL21(DE3)
-
protein expression of Y63 using GAL promoter of pYes2NT vector in yeast
-
recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL(DE3)
-
recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
the gene encoding the enzyme is cloned and sequenced, it shows high sequence homology to zinc-containing alcohol dehydrogenases and L-threonine dehydrogenases with binding motifs of catalytic zinc and NADP+