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177
(R)-2-butanol
-
pH 7.5, 37°C
123
(S)-2-butanol
-
pH 7.5, 37°C
3.47 - 6.7
1,2-naphthoquinone
0.25 - 0.55
1,4-Naphthoquinone
0.00027 - 0.00062
1-(3-methyl-1,4-dioxidoquinoxalin-2-yl)ethanone
2.72 - 2.89
1-(3-methyl-4-oxidoquinoxalin-2-yl)ethanone
17
1-phenylisatin
-
pH 6.0
16.4
2,2,2-trifluoroacetophenone
pH 9.0, 70°C, recombinant enzyme
26
2,3-Butanediol
-
pH 7.5, 37°C
23
2,3-heptanedione
-
pH 6.0
0.32 - 24
2,3-Hexanedione
10.7 - 36.7
2,3-Pentanedione
149
2-butanol
-
pH 7.5, 37°C
86
2-butanone
-
pH 7.5, 37°C
1.13 - 6.76
2-hydroxyacetophenone
5
2-Pentanol
-
pH 7.5, 37°C
18
2-Pentanone
-
pH 7.5, 37°C
243
2-propanol
-
pH 7.5, 37°C
6.75
3-glutathionyl-4-hydroxynonanal
-
pH 8.4, 37°C
30.5
3-glutathionyl-hexanal
pH 8.4, 25°C
30.8
3-glutathionyl-nonanal
pH 8.4, 25°C
190.8
3-glutathionyl-propanal
pH 8.4, 25°C
1 - 2.02
4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone
0.009167 - 17
4-benzoylpyridine
17
4-hexanoylpyridine
-
pH 6.0
0.0098 - 3.48
4-nitrobenzaldehyde
220
4-oxonon-2-enal
-
pH 7.4, 37°C, recombinant enzyme
1.27 - 36.2
9,10-phenanthrenequinone
66.7
9,10-phenantrenequinone
at pH 8.4 and 37°C
-
0.07
9-cis-retinal
-
pH 7.4
13
acenaphthenequinone
-
pH 6.0
183
acetoin
-
pH 7.5, 37°C
0.18
acetone
pH 6.5, 25°C
0.08
all-trans-retinal
-
pH 7.4
0.01
all-trans-retinol
-
pH 7.4
32
benzoylformic acid ethyl ester
-
pH 6.0
6
Cycloheptanone
-
pH 7.5, 37°C
5
Cyclohexanol
-
pH 7.5, 37°C
86
cyclohexanone
-
pH 7.5, 37°C
106
Cyclopentanol
-
pH 7.5, 37°C
80
Cyclopentanone
-
pH 7.5, 37°C
609
diacetyl
-
pH 7.5, 37°C
0.0014 - 0.21
doxorubicin
0.45
estrone
pH 6.5, 25°C
12.5
ethyl 2-methylacetoacetate
pH 9.0, 70°C, recombinant enzyme
11.2
ethyl 2-oxo-4-phenylbutyrate
pH 9.0, 70°C, recombinant enzyme
1.05
ethyl 4-chloro-3-oxobutanoate
pH 6.0, 50°C
8.6
glutathione-4-oxonon-2-enal
-
pH 7.4, 37°C, recombinant enzyme
30.8
glutathionyl-nonanal
at pH 8.4 and 37°C
-
10
heptanophenone
-
pH 6.0
25
hexanophenone
-
pH 6.0
23.8
methylglyoxal
pH 7.0, 25°C
15
n-butyrophenone
-
pH 6.0
39.3
Phenylglyoxal
pH 7.0, 25°C
129
propanone
-
pH 7.5, 37°C
0.68 - 25
prostaglandin E2
3.5
S-1-phenyl-1-butanol
-
pH 7.4
2.02 - 20.45
S-nitrosoglutathione
0.6
testosterone
pH 6.5, 25°C
11
valerophenone
-
pH 6.0
3.47
1,2-naphthoquinone
wild-type, pH 7.4, 25°C
3.47
1,2-naphthoquinone
wild-type, pH 7.4, 37°C
6.7
1,2-naphthoquinone
after treatment with S-nitrosoglutathione, pH 7.4, 25°C
6.7
1,2-naphthoquinone
enzyme modified by S-nitrosoglutathione, pH 7.4, 37°C
0.25
1,4-Naphthoquinone
after treatment with S-nitrosoglutathione, pH 7.4, 25°C
0.28
1,4-Naphthoquinone
enzyme modified by S-nitrosoglutathione, pH 7.4, 37°C
0.55
1,4-Naphthoquinone
wild-type, pH 7.4, 25°C
0.55
1,4-Naphthoquinone
wild-type, pH 7.4, 37°C
0.00027
1-(3-methyl-1,4-dioxidoquinoxalin-2-yl)ethanone
pH 7.4, 37°C
0.00062
1-(3-methyl-1,4-dioxidoquinoxalin-2-yl)ethanone
pH 7.4, 37°C
2.72
1-(3-methyl-4-oxidoquinoxalin-2-yl)ethanone
pH 7.4, 37°C
2.89
1-(3-methyl-4-oxidoquinoxalin-2-yl)ethanone
pH 7.4, 37°C
6.8
16-ketoestrone
-
pH 7.4
20
16-ketoestrone
-
pH 6.0
0.32
2,3-Hexanedione
after treatment with S-nitrosoglutathione, pH 7.4, 25°C
1.52
2,3-Hexanedione
enzyme modified by S-nitrosoglutathione, pH 7.4, 37°C
5.13
2,3-Hexanedione
wild-type, pH 7.4, 25°C
5.13
2,3-Hexanedione
wild-type, pH 7.4, 37°C
24
2,3-Hexanedione
-
pH 6.0
10.7
2,3-Pentanedione
pH 7.0, 25°C
23
2,3-Pentanedione
-
pH 6.0
36.7
2,3-Pentanedione
pH 7.0, 25°C
1.13
2-hydroxyacetophenone
mutant S172T, with NADH as cofactor, at 35°C in 100 mM acetate buffer (pH 5.0)
1.21
2-hydroxyacetophenone
mutant S67D/H68D, NADPH as cofactor
1.21
2-hydroxyacetophenone
mutant S67D/H68D, with NADPH as cofactor, at 35°C in 100 mM acetate buffer (pH 5.0)
1.22
2-hydroxyacetophenone
mutant V270D, with NADH as cofactor, at 35°C in 100 mM acetate buffer (pH 5.0)
1.24
2-hydroxyacetophenone
mutant DELTA31, with NADH as cofactor, at 35°C in 100 mM acetate buffer (pH 5.0)
1.26
2-hydroxyacetophenone
mutant S67D/H68D, NADH as cofactor
1.26
2-hydroxyacetophenone
mutant S67D/H68D, with NADH as cofactor, at 35°C in 100 mM acetate buffer (pH 5.0)
1.32
2-hydroxyacetophenone
wild-type, NADH as cofactor
1.32
2-hydroxyacetophenone
wild-type, with NADH as cofactor, at 35°C in 100 mM acetate buffer (pH 5.0)
1.34
2-hydroxyacetophenone
mutant V270D, with NADPH as cofactor, at 35°C in 100 mM acetate buffer (pH 5.0)
1.36
2-hydroxyacetophenone
mutant S172T, with NADPH as cofactor, at 35°C in 100 mM acetate buffer (pH 5.0)
1.39
2-hydroxyacetophenone
mutant DELTA31, with NADPH as cofactor, at 35°C in 100 mM acetate buffer (pH 5.0)
1.48
2-hydroxyacetophenone
wild-type, NADPH as cofactor
1.48
2-hydroxyacetophenone
wild-type, with NADPH as cofactor, at 35°C in 100 mM acetate buffer (pH 5.0)
1.72
2-hydroxyacetophenone
mutant H68D/P69D, NADPH as cofactor
1.95
2-hydroxyacetophenone
mutant H68D, NADPH as cofactor
2.23
2-hydroxyacetophenone
mutant P69D, NADPH as cofactor
2.32
2-hydroxyacetophenone
mutant S67D/P69D, NADPH as cofactor
2.35
2-hydroxyacetophenone
mutant S67D, NADPH as cofactor
6.37
2-hydroxyacetophenone
mutant H68D/P69D, NADH as cofactor
6.54
2-hydroxyacetophenone
mutant P69D, NADH as cofactor
6.58
2-hydroxyacetophenone
mutant H68D, NADH as cofactor
6.63
2-hydroxyacetophenone
mutant S67D, NADH as cofactor
6.76
2-hydroxyacetophenone
mutant S67D/P69D, NADH as cofactor
1
4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone
enzyme modified by S-nitrosoglutathione, pH 7.4, 37°C
2.02
4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone
wild-type, pH 7.4, 37°C
0.009167
4-benzoylpyridine
-
mutant P230W
0.0103
4-benzoylpyridine
-
wild-type
0.012
4-benzoylpyridine
pH 6.5, 25°C
0.053
4-benzoylpyridine
at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C
0.063
4-benzoylpyridine
-
wild-type
0.065
4-benzoylpyridine
-
mutant W230P
0.183
4-benzoylpyridine
mutant W230P
0.23
4-benzoylpyridine
wild-type
0.25
4-benzoylpyridine
enzyme modified by S-nitrosoglutathione, pH 7.4, 37°C
0.3
4-benzoylpyridine
after treatment with S-nitrosoglutathione, pH 7.4, 25°C
1.03
4-benzoylpyridine
at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C
1.2
4-benzoylpyridine
wild-type, pH 7.4, 25°C
1.2
4-benzoylpyridine
wild-type, pH 7.4, 37°C
1.967
4-benzoylpyridine
at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C
17
4-benzoylpyridine
-
pH 6.0
0.0098
4-nitrobenzaldehyde
pH 6.5, 25°C
0.0283
4-nitrobenzaldehyde
at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C
0.3
4-nitrobenzaldehyde
at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C
1.087
4-nitrobenzaldehyde
at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C
3.48
4-nitrobenzaldehyde
pH 6.5, 25°C
1.27
9,10-phenanthrenequinone
pH 6.0, 50°C
2.33
9,10-phenanthrenequinone
pH 6.5, 25°C
3.57
9,10-phenanthrenequinone
wild-type, pH 7.4, 25°C
3.57
9,10-phenanthrenequinone
wild-type, pH 7.4, 37°C
5.25
9,10-phenanthrenequinone
wild-type, pH 7.4, temperature not specified in the publication
6.7
9,10-phenanthrenequinone
after treatment with S-nitrosoglutathione, pH 7.4, 25°C
9.77
9,10-phenanthrenequinone
enzyme modified by S-nitrosoglutathione, pH 7.4, 37°C
20
9,10-phenanthrenequinone
-
pH 6.0
36.2
9,10-phenanthrenequinone
mutant C227S, pH 7.4, temperature not specified in the publication
0.48
daunorubicin
enzyme modified by S-nitrosoglutathione, pH 7.4, 37°C
1.12
daunorubicin
J9P7P2
mutant D218V, pH 7.4, 37°C
1.19
daunorubicin
single nucleotide polymorphism V88I mutation
1.27
daunorubicin
wild-type, pH 7.4, 37°C
1.94
daunorubicin
wild-type
2.02
daunorubicin
single nucleotide polymorphism P131S mutation
3.52
daunorubicin
J9P7P2
wild-type, pH 7.4, 37°C
61
daunorubicin
-
mutant V88I, 37°C, pH 7.4
91
daunorubicin
-
wild-type, 37°C, pH 7.4
0.0014
doxorubicin
isoform Cbr3, at pH 7.0 and 37°C
0.015
doxorubicin
isoform Cbr1, at pH 7.0 and 37°C
0.15
doxorubicin
single nucleotide polymorphism V88I mutation
0.18
doxorubicin
single nucleotide polymorphism P131S mutation
0.21
doxorubicin
wild-type
0.17
isatin
wild-type, pH 7.4, 25°C
0.17
isatin
wild-type, pH 7.4, 37°C
0.3
isatin
enzyme modified by S-nitrosoglutathione, pH 7.4, 37°C
0.5167
isatin
at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C
0.583
isatin
at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C
1.225
isatin
at 100 mM sodium phosphate buffer, pH 6.5, 0.065 mM NADPH, 25°C
9.77
isatin
after treatment with S-nitrosoglutathione, pH 7.4, 25°C
0.0092
menadione
pH 6.5, 25°C
0.011
menadione
-
wild-type
0.01217
menadione
-
mutant P230W
0.04167
menadione
-
mutant W230P
0.0483
menadione
-
wild-type
0.15
menadione
pH 7.4, 37°C
0.19
menadione
single nucleotide polymorphism V88I mutation
0.27
menadione
pH 6.5, 25°C
0.28
menadione
after treatment with S-nitrosoglutathione, pH 7.4, 25°C
0.32
menadione
enzyme modified by S-nitrosoglutathione, pH 7.4, 37°C
0.33
menadione
single nucleotide polymorphism P131S mutation
0.68
menadione
wild-type, pH 7.4, 25°C
0.68
menadione
wild-type, pH 7.4, 37°C
1.12
menadione
J9P7P2
mutant D218V, pH 7.4, 37°C
4.17
menadione
pH 7.4, 37°C
8.48
menadione
J9P7P2
wild-type, pH 7.4, 37°C
9.9
menadione
-
pH 7.4, 37°C, recombinant isozyme CBR V244
20.5
menadione
-
pH 7.4, 37°C, recombinant isozyme CBR M244
85
menadione
-
mutant V88I, 37°C, pH 7.4
110
menadione
-
wild-type, 37°C, pH 7.4
246
menadione
-
pH 7.4, 37°C, recombinant enzyme
2.5
methyl glyoxal
pH 6.0, 25°C
4
methyl glyoxal
pH 6.0, 25°C
1.13
NADH
mutant S172T, pH 7.5, 35°C
1.22
NADH
mutant V270D, pH 7.5, 35°C
1.24
NADH
deletion mutant lacking 31 N-terminal amino acids, pH 7.5, 35°C
1.26
NADH
mutant S67D/H68D, pH 7.5, 35°C
1.32
NADH
wild-type, pH 7.5, 35°C
64
NADH
-
pH 6.5, 30°C, recombinant mutant S1M1
120
NADH
-
pH 6.5, 30°C, recombinant mutant S1M4
0.0001
NADPH
isoform Cbr3, at pH 7.0 and 37°C
0.036
NADPH
isoform Cbr1, at pH 7.0 and 37°C
1.21
NADPH
mutant S67D/H68D, pH 7.5, 35°C
1.34
NADPH
mutant V270D, pH 7.5, 35°C
1.36
NADPH
mutant S172T, pH 7.5, 35°C
1.39
NADPH
deletion mutant lacking 31 N-terminal amino acids, pH 7.5, 35°C
1.48
NADPH
wild-type, pH 7.5, 35°C
16.3
NADPH
-
pH 7.4, 37°C, recombinant isozyme CBR V244
26.7
NADPH
-
pH 7.4, 37°C, recombinant isozyme CBR M244
149
NADPH
-
mutant V88I, 37°C, pH 7.4
179
NADPH
-
wild-type, 37°C, pH 7.4
870
NADPH
-
pH 6.5, 30°C, recombinant wild-type enzyme
0.38
oracin
enzyme modified by S-nitrosoglutathione, pH 7.4, 37°C
1.67
oracin
wild-type, pH 7.4, 37°C
2.5
p-toluquinone
pH 6.0, 50°C
3.28
p-toluquinone
pH 6.5, 25°C
0.68
prostaglandin E2
pH 6.5, 25°C
18
prostaglandin E2
-
mutant V88I, 37°C, pH 7.4
25
prostaglandin E2
-
wild-type, 37°C, pH 7.4
2.02
S-nitrosoglutathione
mutant C227S, pH 7.4, temperature not specified in the publication
2.62
S-nitrosoglutathione
wild-type, pH 7.4, temperature not specified in the publication
5.05
S-nitrosoglutathione
mutant D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244P, pH 7.4, temperature not specified in the publication
10.83
S-nitrosoglutathione
mutant P230W/D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244PpH 7.4, temperature not specified in the publication
20.45
S-nitrosoglutathione
mutant Q142M/C143S/P230W/D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244P/H270S, pH 7.4, temperature not specified in the publication