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101
(2E)-but-2-en-1-ol
pH 8.0, 55°C
405
(2E)-but-2-enal
pH 6.5, 65°C
1.01 - 1.9
(R)-2-pentanol
61.4
(S)-(+)-1-indanol
-
65°C
57
(S)-(+)-alpha-tetraol
-
65°C
1.1
(S)-(-)-1-phenylethanol
-
65°C
13.7 - 61.4
(S)-1-indanol
1.1
(S)-1-phenylethanol
-
pH 10.5, 65°C
0.87 - 6.7
(S)-2-pentanol
2.1 - 57
(S)-alpha-tetralol
0.041
1,3-Propanediol
pH 7.5, 23°C
1.98
1,4-Butanediol
pH 10, 60°C
17.1
1-phenyl-1,2-propandione
-
pH 10.5, 65°C
3.1 - 17.1
1-phenyl-1,2-propanedione
35.7
1-phenylethanol
pH 9.0, 70°C
0.021
11-cis-retinal
-
reduction with NADH
0.022
11-cis-retinol
-
oxidation with NAD+
1.03 - 20
12-hydroxydodecanoate
0.333
12-Hydroxydodecanoic acid
-
-
27.6 - 34.3
12-hydroxylauric acid methyl ester
308
12-oxolauric acid methyl ester
wild-type, pH 8.0, 60°C
0.018
13-cis-retinal
-
reduction with NADH
0.018
13-cis-retinol
-
oxidation with NAD+
6.17
16-hydroxyhexadecanoate
-
-
3.2
2',3',4',5',6'-pentafluoroacetophenone
65°C, pH 5.0
1.7 - 25.5
2,2,2-trifluoroacetophenone
0.65
2,2-dichloroacetophenone
65°C, pH 5.0
0.03 - 43.8
2,3-Butanediol
0.4
2-decanone
pH 8.0, 60°C
1.4
2-dehydro-3-deoxy-D-gluconate
at pH 7.9 and 25°C
2.83
2-deoxy-D-ribose
-
-
1.6
2-ethoxyethanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.73
2-Heptanone
pH 8.0, 60°C
1.08
2-Hexanone
pH 8.0, 60°C
0.7 - 1
2-nonanone
pH 8.0, 60°C
0.74
2-Octanone
pH 8.0, 60°C
0.6
2-Pentanol
pH 8.0, 60°C
0.0713 - 0.77
2-Pentanone
0.22 - 1.22
3,4-dihydro-retinal
0.088 - 2.5
3,4-dihydro-retinol
1.2 - 2
3-bromobenzyl alcohol
1.2
3-bromobenzylalcohol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.2 - 1.2
3-hydroxy-2-butanone
3.1
3-Methoxybenzaldehyde
-
65°C
1.4 - 16
3-Methoxybenzyl alcohol
1.2 - 1.4
3-methoxybenzylalcohol
0.08 - 0.27
3-methyl-1-butanol
7.72
3-methyl-cyclohexanol
-
-
1.22
3-methylbutan-2-one
-
-
2.17 - 3.8
3-methylcyclohexanone
2.05
3-oxo-5beta-androstan-17beta-ol
-
-
7.5
3-Phenyl-1-propanol
-
-
0.683
3-Pyridylcarbinol
-
pH 7.5, anodic enzyme form
380
4-amino-1-butanol
pH 9.0, 25°C, recombinant enzyme
1.1 - 3.5
4-bromobenzyl alcohol
1.1
4-bromobenzylalcohol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.7
4-carboxybenzaldehyde
145 - 432
4-chloroacetophenone
0.092 - 34.2
4-hydroxy-retinol
0.6 - 20.5
4-methoxybenzaldehyde
0.37 - 25.5
4-methoxybenzyl alcohol
1.5
4-methoxybenzylalcohol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.071
4-methoxyphenylacetone
pH 8.0, 60°C
6.7 - 18.6
4-Methyl-1-pentanol
0.9
5beta-Pregnan-21-ol-3,20-dione hemisuccinate
-
-
0.023
9-cis-retinal
-
reduction with NADH
0.025
9-cis-retinol
-
oxidation with NAD+
13.7
acetoin
pH 6.0, 70°C
0.35 - 1.9
all-trans retinol
0.018 - 2.5
all-trans-retinal
0.028 - 1.55
all-trans-retinol
48.1
alpha-tetralol
-
65°C
0.35 - 93.3
benzyl alcohol
1.4
benzylalcohol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
55
cinnamaldehyde
-
pH 7.7, 60°C
43 - 133
cinnamyl alcohol
0.98
cis-decahydro-1-naphthol
65°C, pH 8.0
0.167 - 15.9
Cyclohexanol
0.18
D-allonate
at pH 7.9 and 25°C
0.19
D-altronate
at pH 7.9 and 25°C
0.0019
D-arabitol
at pH 7.9 and 25°C
0.98
D-gluconate
at pH 7.9 and 25°C
0.53
D-mannonate
at pH 7.9 and 25°C
0.0052
D-ribonate
at pH 7.9 and 25°C
0.0052
D-sorbitol
at pH 7.9 and 25°C
0.021
D-talonate
at pH 7.9 and 25°C
0.032
D-xylonate
at pH 7.9 and 25°C
26
ethyl 3-methyl-2-oxobutyrate
65°C, pH 5.0
1.9 - 50.1
ethyl benzoylformate
50.1
ethyl oxo(phenyl)acetate
-
pH 10.5, 65°C
3.97
ethyl pyruvate
-
pH 6.5, 50°C, recombinant enzyme
0.75 - 4.5
ethylene glycol
1.28
formaldehyde
-
pH 7.0, 30°C
93.13
Glutaraldehyde
pH 6, 60°C
15.44 - 18.65
glycolaldehyde
0.076
iso-propanol
pH 7.5, 23°C
16.6
isoborneol
65°C, pH 10.5
0.82 - 29
Isobutyraldehyde
6.2
methyl benzoylformate
65°C, pH 5.5
9.2
methyl o-chlorobenzoylformate
38.1
methyl oxo(phenyl)acetate
-
pH 10.5, 65°C
38.1
methylbenzoylformate
-
65°C
2.63 - 8.58
methylcrotonyl alcohol
1.85
octan-1-ol
-
enzyme form ADH-3
0.24
p-nitrophenyl octanoate
-
-
2.92
pentan-1-ol
-
enzyme form ADH-3
202 - 303
phenylacetaldehyde
0.73
propanal
-
pH 7.0, 30°C
0.026
tert-butanol
pH 7.5, 23°C
0.072
tert-butyl acetoacetate
pH 8.0, 60°C
9.6
tetralin-1-ol
65°C, pH 10.5
0.21 - 18.9
trans-cinnamaldehyde
0.467 - 8.67
Vanillyl alcohol
additional information
additional information
-
7.1
(R)-1-indanol
pH 8.0, 65°C
7.1
(R)-1-indanol
65°C, pH 8.0
1
(R)-2-butanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
1.7
(R)-2-butanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
1.01
(R)-2-pentanol
-
-
1.1
(R)-2-pentanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
1.9
(R)-2-pentanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
13.5
(R)-alpha-tetralol
pH 8.0, 65°C
13.5
(R)-alpha-tetralol
65°C, pH 8.0
13.7
(S)-1-indanol
pH 8.0, 65°C
13.7
(S)-1-indanol
65°C, pH 8.0
61.4
(S)-1-indanol
-
pH 10.5, 65°C
1
(S)-2-butanol
-
-
1.2
(S)-2-butanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
4.8
(S)-2-butanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.87
(S)-2-pentanol
-
-
2.6
(S)-2-pentanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
6.7
(S)-2-pentanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
2.1
(S)-alpha-tetralol
pH 8.0, 65°C
2.1
(S)-alpha-tetralol
65°C, pH 8.0
57
(S)-alpha-tetralol
-
pH 10.5, 65°C
0.004
1-butanol
pH 7.5, 23°C
0.41
1-butanol
pH 8.0, 60°C
0.833
1-butanol
-
isoenzyme ADH-3, pH 10.0
3.1
1-butanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
15.1
1-butanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
48.8
1-butanol
-
isoenzyme ADH-1, pH 10.0
0.51
1-Heptanol
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
1.6
1-Heptanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
11.9
1-Heptanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.37
1-Hexanol
pH 8.0, 60°C
0.42
1-Hexanol
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
2
1-Hexanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
13.3
1-Hexanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
6.2
1-Indanol
65°C, pH 10.5
8.3
1-Indanone
-
65°C
8.3
1-Indanone
-
pH 10.5, 65°C
1
1-Octanol
-
isoenzyme ADH-3, pH 10.0
2.33
1-Octanol
-
isoenzyme ADH-2, pH 10.0
60.8
1-Octanol
-
isoenzyme ADH-1, pH 10.0
0.183
1-Pentanol
-
-
0.45
1-Pentanol
pH 8.0, 60°C
0.53
1-Pentanol
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
1.17
1-Pentanol
-
isoenzyme ADH-3, pH 10.0
2.5
1-Pentanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
3.53
1-Pentanol
-
isoenzyme ADH-2, pH 10.0
16.3
1-Pentanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
48.8
1-Pentanol
-
isoenzyme ADH-1, pH 10.0
3.1
1-phenyl-1,2-propanedione
65°C, pH 5.5
5.3
1-phenyl-1,2-propanedione
65°C, pH 5.0
17.1
1-phenyl-1,2-propanedione
-
65°C
0.019
1-propanol
pH 7.5, 23°C
0.26
1-propanol
pH 8.0, 60°C
1.2
1-propanol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
1.2
1-propanol
-
native enzyme, at 55°C
2.2
1-propanol
-
carboxymethylated enzyme, at 55°C
2.5
1-propanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
2.6
1-propanol
-
wild type enzyme, at 65°C
15.7
1-propanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
16.6
1-propanol
-
mutant enzyme N249Y, at 65°C
1.03
12-hydroxydodecanoate
-
isoenzyme TT-ADH
1.27
12-hydroxydodecanoate
-
isoenzyme ADH-3, pH 10.0
1.45
12-hydroxydodecanoate
-
enzyme form ADH-3
2.2
12-hydroxydodecanoate
-
isoenzyme BB-ADH
2.43
12-hydroxydodecanoate
-
-
2.77
12-hydroxydodecanoate
-
isoenzyme AA-ADH
2.95
12-hydroxydodecanoate
-
-
3.03
12-hydroxydodecanoate
-
-
3.83
12-hydroxydodecanoate
-
isoenzyme ADH-12, pH 10.0
4
12-hydroxydodecanoate
-
-
20
12-hydroxydodecanoate
-
isoenzyme ADH-1, pH 10.0
27.6
12-hydroxylauric acid methyl ester
wild-type, pH 8.0, 60°C
34.3
12-hydroxylauric acid methyl ester
mutant C257L, pH 8.0, 60°C
1.7
2,2,2-trifluoroacetophenone
65°C, pH 5.0
10
2,2,2-trifluoroacetophenone
65°C, pH 5.5
25.5
2,2,2-trifluoroacetophenone
-
65°C
25.5
2,2,2-trifluoroacetophenone
-
pH 10.5, 65°C
0.03
2,3-Butanediol
-
45°C, pH 8.8, cosubstrate: NADP+, wild-type enzyme
0.073
2,3-Butanediol
-
pH 8.8, 45°C, wild-type enzyme, cofactor: NAP+
0.29
2,3-Butanediol
-
pH 8.8, 45°C, mutant enzyme G211S, cofactor: NADP+
0.43
2,3-Butanediol
-
pH 8.8, 45°C, mutant enzyme G211InsC, cofactor: NAD+
0.52
2,3-Butanediol
-
pH 8.8, 45°C, mutant enzyme G211InsG, cofactor: NADP+
0.86
2,3-Butanediol
-
pH 8.8, 45°C, mutant enzyme G211InsC, cofactor: NADP+
0.92
2,3-Butanediol
-
pH 8.8, 45°C, mutant enzyme G211C, cofactor: NADP+
1
2,3-Butanediol
-
45°C, pH 8.8, cosubstrate: NAD+, wild-type enzyme
1.1
2,3-Butanediol
-
pH 8.8, 45°C, mutant enzyme G211C, cofactor: NAD+
1.7
2,3-Butanediol
-
pH 8.8, 45°C, mutant enzyme G211InsA, cofactor: NAD+
2.1
2,3-Butanediol
-
pH 8.8, 45°C, mutant enzyme G211InsA, cofactor: NADP+
2.1
2,3-Butanediol
-
pH 8.8, 45°C, mutant enzyme G211InsS, cofactor: NADP+
2.6
2,3-Butanediol
-
pH 8.8, 45°C, wild-type enzyme, cofactor: NAD+
4
2,3-Butanediol
-
pH 8.8, 45°C, mutant enzyme G211S, cofactor: NAD+
4.7
2,3-Butanediol
-
45°C, pH 8.8, cosubstrate: NADP+, mutant enzyme K249G/H255R
6.5
2,3-Butanediol
-
pH 8.8, 45°C, mutant enzyme G211InsG, cofactor: NAD+
6.7
2,3-Butanediol
-
pH 8.8, 45°C, mutant enzyme G211InsS, cofactor: NAD+
15
2,3-Butanediol
-
45°C, pH 8.8, cosubstrate: NAD+, mutant enzyme K249G/H255R
43.8
2,3-Butanediol
pH 9.0, 70°C
0.094
2-butanol
pH 7.5, 23°C
0.48
2-butanol
pH 8.0, 60°C
1.67
2-Buten-1-ol
-
isoenzyme ADH-3, pH 10.0
5.83
2-Buten-1-ol
-
isoenzyme ADH-2, pH 10.0
213
2-Buten-1-ol
-
isoenzyme ADH-1, pH 10.0
0.0713
2-Pentanone
-
-
0.77
2-Pentanone
pH 8.0, 60°C
0.24
2-propanol
pH 8.0, 60°C
0.25
2-propanol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.25
2-propanol
-
native enzyme, at 55°C
0.45
2-propanol
-
carboxymethylated enzyme, at 55°C
287
2-propanol
-
pH 7.7, 60°C
0.22
3,4-dihydro-retinal
-
pH 7.5, 25°C, isozyme ADH1B2
1.22
3,4-dihydro-retinal
-
pH 7.5, 25°C, isozyme ADH4
0.088
3,4-dihydro-retinol
-
pH 7.5, 25°C, isozyme ADH1B2
2.5
3,4-dihydro-retinol
-
pH 7.5, 25°C, isozyme ADH4
1.2
3-bromobenzyl alcohol
-
native enzyme, at 55°C
2
3-bromobenzyl alcohol
-
carboxymethylated enzyme, at 55°C
0.2
3-hydroxy-2-butanone
-
45°C, pH 6.1, cosubstrate: NADPH, wild-type enzyme
0.36
3-hydroxy-2-butanone
-
45°C, pH 6.1, cosubstrate: NADH, wild-type enzyme
1.1
3-hydroxy-2-butanone
-
45°C, pH 6.1, cosubstrate: NADH, mutant enzyme K249G/H255R
1.2
3-hydroxy-2-butanone
-
45°C, pH 6.1, cosubstrate: NADPH, mutant enzyme K249G/H255R
1.4
3-Methoxybenzyl alcohol
-
native enzyme, at 55°C
16
3-Methoxybenzyl alcohol
-
carboxymethylated enzyme, at 55°C
1.2
3-methoxybenzylalcohol
-
in the presence of 5 mM benzylalcohol, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
1.4
3-methoxybenzylalcohol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.08
3-methyl-1-butanol
-
mutant enzyme W54L
0.27
3-methyl-1-butanol
-
wild-type enzyme Adh 1
2.17
3-methylcyclohexanone
-
-
3.8
3-methylcyclohexanone
65°C, pH 5.5
1.5
3-Pentanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
1.1
4-bromobenzyl alcohol
-
native enzyme, at 55°C
3.5
4-bromobenzyl alcohol
-
carboxymethylated enzyme, at 55°C
0.7
4-carboxybenzaldehyde
-
in 50 mM Tris-HCl (pH 7.5) containing 0.2 mM NADH + H+, at 65°C
0.7
4-carboxybenzaldehyde
-
native enzyme, at 55°C
145
4-chloroacetophenone
-
pH 6.0, 37°C, recombinant enzyme expressed from Hansenula polymorpha
230
4-chloroacetophenone
-
pH 6.0, 37°C, recombinant enzyme expressed from Saccharomyces cerevisiae
432
4-chloroacetophenone
-
pH 6.0, 37°C, recombinant enzyme expressed from Arxula adeninivorans
0.092
4-hydroxy-retinol
-
pH 7.5, 25°C, isozyme ADH1B1
2.83
4-hydroxy-retinol
-
pH 7.5, 25°C, isozyme ADH1B2
34.2
4-hydroxy-retinol
-
pH 7.5, 25°C, isozyme ADH4
0.6
4-methoxybenzaldehyde
-
in 50 mM Tris-HCl (pH 7.5) containing 0.2 mM NADH + H+, at 65°C
0.6
4-methoxybenzaldehyde
-
native enzyme, at 55°C
3.1
4-methoxybenzaldehyde
-
pH 6.0, 65°C
3.3
4-methoxybenzaldehyde
-
wild type enzyme, at 65°C
20.5
4-methoxybenzaldehyde
-
mutant enzyme N249Y, at 65°C
0.37
4-methoxybenzyl alcohol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.6
4-methoxybenzyl alcohol
pH 8.0, 60°C
1.5
4-methoxybenzyl alcohol
-
native enzyme, at 55°C
1.6
4-methoxybenzyl alcohol
-
65°C
1.6
4-methoxybenzyl alcohol
-
pH 10.5, 65°C
2.9
4-methoxybenzyl alcohol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
3.3
4-methoxybenzyl alcohol
-
wild type enzyme, at 65°C
16.8
4-methoxybenzyl alcohol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
20.5
4-methoxybenzyl alcohol
-
carboxymethylated enzyme, at 55°C
25.5
4-methoxybenzyl alcohol
-
mutant enzyme N249Y, at 65°C
6.7
4-Methyl-1-pentanol
-
wild-type enzyme Adh 1
18.6
4-Methyl-1-pentanol
-
mutant enzyme W54L
4.6
4-nitrobenzaldehyde
-
in 50 mM Tris-HCl (pH 7.5) containing 0.2 mM NADH + H+, at 65°C
4.6
4-nitrobenzaldehyde
-
native enzyme, at 55°C
0.167
4-oxo-retinal
-
pH 7.5, 25°C, isozyme ADH1B1
7.83
4-oxo-retinal
-
pH 7.5, 25°C, isozyme ADH1B2
20
4-oxo-retinal
-
pH 7.5, 25°C, isozyme ADH4
0.3
acetaldehyde
-
40°C, pH 9.0, 50 mM Tris-HCl, 4 M NaCl
2.5
acetaldehyde
-
pH 7.0, 30°C
3.33
acetaldehyde
-
pyrazole-insensitive enzyme, pH 7.0
5.4
acetaldehyde
-
70°C, pH 9.0, 50 mM Tris-HCl, 4 M NaCl
16.7
acetaldehyde
-
pyrazole-sensitive enzyme, pH 7.0
53
acetaldehyde
pH 7.0, temperature not specified in the publication
83
acetaldehyde
-
wild-type, 60°C, pH 6.0, presence of 0.1 mM Zn2+
283
acetaldehyde
pH 7.0, temperature not specified in the publication
313.14
acetaldehyde
pH 7.0, 30°C, with NADH
350
acetaldehyde
-
alcohol dehydrogenase domain, 60°C, pH 6.0, presence of 0.1 mM Zn2+
404.8
acetaldehyde
pH 8, 60°C
681
acetaldehyde
wild-type, pH 8.0, 60°C
3500
acetaldehyde
pH 6.0, 22°C, recombinant enzyme
0.317
acetone
-
-
211
acetophenone
-
pH 6.0, 37°C, recombinant enzyme expressed from Hansenula polymorpha
259
acetophenone
-
pH 6.0, 37°C, recombinant enzyme expressed from Saccharomyces cerevisiae
288
acetophenone
-
pH 6.0, 37°C, recombinant enzyme expressed from Arxula adeninivorans
0.35
all-trans retinol
-
isoenzyme AA-ADH
1.3
all-trans retinol
-
-
1.3
all-trans retinol
-
isoenzyme TT-ADH
1.9
all-trans retinol
-
isoenzyme BB-ADH
0.018
all-trans-retinal
-
pH 7.5, 25°C, isozyme ADH1B1
0.022
all-trans-retinal
-
oxidation with NAD+
0.55
all-trans-retinal
-
pH 7.5, 25°C, isozyme ADH1B2
1.83
all-trans-retinal
-
pH 7.5, 25°C, isozyme ADH4
2.5
all-trans-retinal
-
reduction with NADH
0.028
all-trans-retinol
-
pH 7.5, 25°C, isozyme ADH1B1
0.25
all-trans-retinol
-
pH 7.5, 25°C, isozyme ADH1B2
0.4
all-trans-retinol
-
-
0.9
all-trans-retinol
-
pH 7.5, 25°C, isozyme ADH4
1.55
all-trans-retinol
-
oxidation with NAD+
7.7
alpha-tetralone
-
65°C
7.7
alpha-tetralone
-
pH 10.5, 65°C
0.36
benzaldehyde
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
1.22
benzaldehyde
pH 8.0, 60°C
1.8
benzaldehyde
-
carboxymethylated enzyme, at 55°C
3.5
benzaldehyde
-
in 50 mM Tris-HCl (pH 7.5) containing 0.2 mM NADH + H+, at 65°C
3.5
benzaldehyde
-
native enzyme, at 55°C
11.3
benzaldehyde
-
wild type enzyme, at 65°C
11.3
benzaldehyde
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
13.1
benzaldehyde
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
25.5
benzaldehyde
-
mutant enzyme N249Y, at 65°C
360
benzaldehyde
pH 6.5, 65°C
0.35
benzyl alcohol
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
1
benzyl alcohol
-
isoenzyme ADH-3, pH 10.0
1.02
benzyl alcohol
pH 8.0, 60°C
1.4
benzyl alcohol
-
native enzyme, at 55°C
2.52
benzyl alcohol
-
enzyme form ADH-3
3.6
benzyl alcohol
-
wild type enzyme, at 65°C
3.6
benzyl alcohol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
3.8
benzyl alcohol
mutant A25Y, pH 7.0, 30°C
4.67
benzyl alcohol
-
isoenzyme alpha,gamma1
5.5
benzyl alcohol
mutant W87A, pH 7.0, 30°C
6.9
benzyl alcohol
wild-type, pH 7.0, 30°C
14
benzyl alcohol
mutant Y25A, pH 7.0, 30°C
14.5
benzyl alcohol
-
carboxymethylated enzyme, at 55°C
20
benzyl alcohol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
21.7
benzyl alcohol
-
mutant enzyme N249Y, at 65°C
24.9
benzyl alcohol
wild-type, pH 7.0, 30°C
89.7
benzyl alcohol
-
isoenzyme ADH-1, pH 10.0
93.3
benzyl alcohol
pH 8.0, 55°C
1.97
butan-1-ol
-
-
2
butan-1-ol
cosubstrate NAD+, pH 7.0, 30°C
7.22
butan-1-ol
-
enzyme form ADH-3
0.31
butan-2-ol
-
mutant enzyme W54L
11.9
butan-2-ol
-
wild-type enzyme Adh 1
1.99
Butanal
-
pH 7.0, 30°C
102
Butanal
cosubstrate NADH, pH 7.0, 30°C
0.0833
butanol
-
pyrazole-insensitive enzyme, pH 7.5
0.35
butanol
-
pyrazole-sensitive enzyme, pH 7.5
0.45
butanol
-
pyrazole-sensitive enzyme and pyrazole-insensitive enzyme, pH 10.0
0.7
butanol
-
pH 7.5, anodic enzyme form
32.7
butanol
-
mutant enzyme W54L
118
butanol
-
wild-type enzyme Adh 1
0.36
Butyraldehyde
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
19.7
Butyraldehyde
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
19.7
Butyraldehyde
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
931
Butyraldehyde
wild-type, pH 8.0, 60°C
317
capronaldehyde
-
pH 6.0, 37°C, recombinant enzyme expressed from Arxula adeninivorans
410
capronaldehyde
-
pH 6.0, 37°C, recombinant enzyme expressed from Hansenula polymorpha
576
capronaldehyde
-
pH 6.0, 37°C, recombinant enzyme expressed from Saccharomyces cerevisiae
43
cinnamyl alcohol
-
pH 7.7, 60°C
98
cinnamyl alcohol
-
mutant enzyme W54L
133
cinnamyl alcohol
-
wild-type enzyme Adh 1
4.4
cycloheptanol
pH 8.0, 65°C
4.4
cycloheptanol
65°C, pH 8.0
7
cycloheptanol
65°C, pH 10.5
0.167
Cyclohexanol
-
-
0.383
Cyclohexanol
-
isoenzyme ADH-2, pH 10.0
0.52
Cyclohexanol
pH 8.0, 60°C
0.65
Cyclohexanol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.65
Cyclohexanol
-
native enzyme, at 55°C
0.75
Cyclohexanol
-
enzyme form ADH-2
1.2
Cyclohexanol
-
carboxymethylated enzyme, at 55°C
1.2
Cyclohexanol
-
wild type enzyme, at 65°C
1.2
Cyclohexanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
1.5
Cyclohexanol
-
isoenzyme ADH-3, pH 10.0
1.75
Cyclohexanol
-
isoenzyme BB-ADH
1.75
Cyclohexanol
-
isoenzyme ADH-1, pH 10.0
2.4
Cyclohexanol
-
isoenzyme AA-ADH
3.55
Cyclohexanol
-
enzyme form ADH-3
8.5
Cyclohexanol
-
isoenzyme TT-ADH
12.4
Cyclohexanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
15.9
Cyclohexanol
-
mutant enzyme N249Y, at 65°C
1.27
cyclohexanone
pH 8.0, 60°C
0.00086
ethanol
-
0.019
ethanol
pH 7.5, 23°C
0.0833
ethanol
-
pyrazole-insensitive enzyme, pH 7.5
0.167
ethanol
-
recombinant allozyme Ile308, pH 7.5, 25°C
0.175
ethanol
-
recombinant allozyme Val308, pH 7.5, 25°C
0.23
ethanol
pH 8.0, 60°C
0.4
ethanol
-
pyrazole-sensitive enzyme, pH 7.5
0.4
ethanol
-
40°C, pH 9.0, 50 mM Tris-HCl, 4 M NaCl
0.45
ethanol
-
pyrazole-insensitive enzyme, pH 10.0
0.5
ethanol
-
in the presence of 3 mM NAD+, in 0.1 M glycine-NaOH, pH 9.2, at 65°C
0.5
ethanol
-
native enzyme, at 55°C
0.567
ethanol
-
pyrazole-sensitive enzyme
0.7
ethanol
-
pH 7.5, anodic enzyme form
0.85
ethanol
-
carboxymethylated enzyme, at 55°C
1
ethanol
-
isoenzyme ADH-3, pH 10.0
1.78
ethanol
-
in the presence of 0.025 mM Zn2+, at pH 9.5 and 37°C
1.92
ethanol
-
recombinant isozyme ADH1, pH 7.5, 25°C
2.47
ethanol
-
in the presence of 0.025 mM Cu2+, at pH 9.5 and 37°C
2.5
ethanol
-
per active site
2.83
ethanol
-
isoenzyme AA-ADH
3.1
ethanol
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
3.2
ethanol
-
pH 7.0, 30°C
3.38
ethanol
-
isoenzyme BB-ADH
3.83
ethanol
-
isoenzyme gamma1,gamma1
4
ethanol
-
isoenzyme alpha,gamma1
4.1
ethanol
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
4.42
ethanol
-
recombinant isozyme ADH1, pH 10.5, 25°C
4.5
ethanol
pH 7.0, temperature not specified in the publication
4.92
ethanol
-
enzyme form ADH-2
6.63
ethanol
-
enzyme form ADH-3
8.2
ethanol
-
70°C, pH 9.0, 50 mM Tris-HCl, 4 M NaCl
9.58
ethanol
-
isoenzyme TT-ADH
10.2
ethanol
-
pH 10.0, anodic enzyme form
31.5
ethanol
recombinant His-tagged wild-type enzyme, pH 7.5, temperature not specified in the publication
33
ethanol
pH 7.0, temperature not specified in the publication
39.9
ethanol
10°C, pH 9.0
41.3
ethanol
-
recombinant isozyme ADH4, pH 7.5, 25°C
52.1
ethanol
recombinant His-tagged mutant C243S, pH 7.5, temperature not specified in the publication
62.48
ethanol
20°C, pH 9.0
62.7
ethanol
-
isoenzyme ADH-1, pH 10.0
102
ethanol
-
mutant enzyme W54L
109.5
ethanol
30°C, pH 9.0
123
ethanol
-
wild-type enzyme Adh 1
142
ethanol
pH 7.0, temperature not specified in the publication
143
ethanol
in 50 mM Tris-HCl, pH 8.0 at 25°C
158
ethanol
pH 7.0, temperature not specified in the publication
160.8
ethanol
40°C, pH 9.0
215
ethanol
-
recombinant isozyme ADH4, pH 10.5, 25°C
251.3
ethanol
-
10°C, pH 9.0
262.3
ethanol
50°C, pH 9.0
305
ethanol
wild-type, pH 8.0, 60°C
308
ethanol
-
wild-type enzyme Adh 1
365
ethanol
mutant C257L, pH 8.0, 60°C
432.3
ethanol
-
20°C, pH 9.0
474.9
ethanol
60°C, pH 9.0
786.1
ethanol
-
30°C, pH 9.0
895.4
ethanol
-
40°C, pH 9.0
3000
ethanol
pH 9.0, 22°C, recombinant enzyme
1.9
ethyl benzoylformate
65°C, pH 5.0
2.35
ethyl benzoylformate
65°C, pH 5.5
50.1
ethyl benzoylformate
-
65°C
0.75
ethylene glycol
-
-
1.58
furfural
-
pH 7.0, 30°C
36.9
furfural
pH 7.0, 30°C, with NADH
15.44
glycolaldehyde
pH 7.0, 30°C, with NADH
18.65
glycolaldehyde
-
pH 7.0, 30°C
0.167
Hexanol
-
-
0.45
Hexanol
-
recombinant isozyme ADH1, pH 7.5, 25°C
3.83
Hexanol
-
recombinant isozyme ADH1, pH 10.5, 25°C
23.9
Hexanol
-
mutant enzyme W54L
29.6
Hexanol
-
wild-type enzyme Adh 1
30.8
Hexanol
-
recombinant isozyme ADH4, pH 7.5, 25°C
86.5
Hexanol
-
recombinant isozyme ADH4, pH 10.5, 25°C
22
isatin
pH 5.5, 65°C
0.82
Isobutyraldehyde
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
18.1
Isobutyraldehyde
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
29
Isobutyraldehyde
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.013
methanol
pH 7.5, 23°C
0.117
methanol
-
pyrazole-sensitive enzyme, pH 7.5
0.217
methanol
-
pyrazole-sensitive enzyme, pH 10.0
9.2
methyl o-chlorobenzoylformate
pH 5.5, 65°C
9.2
methyl o-chlorobenzoylformate
65°C, pH 5.5
2.63
methylcrotonyl alcohol
-
isoenzyme BB-ADH
3
methylcrotonyl alcohol
-
isoenzyme AA-ADH
8.58
methylcrotonyl alcohol
-
isoenzyme TT-ADH
2.58
n-butanol
-
isoenzymee BB-ADH
3.33
n-butanol
-
isoenzyme AA-ADH
7.18
n-butanol
-
isoenzyme TT-ADH
195
n-butanol
wild-type, pH 8.0, 60°C
2.35
n-Hexanol
-
isoenzyme BB-ADH
2.8
n-Hexanol
-
isoenzyme AA-ADH
4.08
n-Hexanol
-
isoenzyme TT-ADH
0.4
NAD+
pH 8.0, 60°C
0.43
NAD+
-
pH 8.8, 45°C, mutant enzyme G211InsC
0.52
NAD+
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
0.717
NAD+
-
pH 7.5, anodic enzyme form
0.84
NAD+
-
pH 10.5, 65°C
1
NAD+
mutant W49F/W87F/V260A, 30°C, pH not specified in the publication
1
NAD+
cosubstrate butan-1-ol, pH 7.0, 30°C
1
NAD+
-
45°C, pH 8.8, cosubstrate: 2,3-butanediol, wild-type enzyme
1.1
NAD+
-
pH 8.8, 45°C, mutant enzyme G211C
1.4
NAD+
-
in 0.1 M glycine-NaOH, pH 9.2, at 65°C
1.4
NAD+
-
native enzyme, at 55°C
1.4
NAD+
mutant W49F/W167Y/V260A, 30°C, pH not specified in the publication
1.7
NAD+
-
pH 8.8, 45°C, mutant enzyme G211InsA
1.9
NAD+
mutant V260A, 30°C, pH not specified in the publication
1.9
NAD+
mutant Y25A/W49F/W167Y/V260A, 30°C, pH not specified in the publication
2.5
NAD+
mutant Y25A/W49F/W167Y, 30°C, pH not specified in the publication
2.5
NAD+
mutant Y25A/W49F/W87F/V260A, 30°C, pH not specified in the publication
2.6
NAD+
-
pH 8.8, 45°C, wild-type enzyme
3
NAD+
mutant W49F/W87F, 30°C, pH not specified in the publication
3.4
NAD+
-
wild type enzyme, at 65°C, in the presence of benzyl alcohol
3.4
NAD+
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
3.4
NAD+
mutant W49F/W167Y, 30°C, pH not specified in the publication
4
NAD+
-
pH 8.8, 45°C, mutant enzyme G211S
4.67
NAD+
-
enzyme form ADH-2
5.1
NAD+
mutant Y25A, 30°C, pH not specified in the publication
6.18
NAD+
-
enzyme form ADH-3
6.3
NAD+
mutant Y25A/W49F/W87F, 30°C, pH not specified in the publication
6.5
NAD+
-
pH 8.8, 45°C, mutant enzyme G211InsG
6.7
NAD+
-
pH 8.8, 45°C, mutant enzyme G211InsS
7.75
NAD+
-
oxidation of ethanol
8
NAD+
wild-type, 30°C, pH not specified in the publication
10.4
NAD+
cosubstrate 1-phenylethanol, pH 9.0, 70°C
15
NAD+
-
45°C, pH 8.8, cosubstrate: 2,3-butanediol, mutant enzyme K249G/H255R
18.5
NAD+
-
carboxymethylated enzyme, at 55°C
21
NAD+
Km above 50 mM, mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
30.8
NAD+
cosubstrate 2,3-butanediol, pH 9.0, 70°C
34.2
NAD+
wild-type, pH 8.0, 60°C
50
NAD+
-
mutant enzyme N249Y, at 65°C, in the presence of benzyl alcohol
443.1
NAD+
cosubstrate ethanol, pH 8, 60°C
0.36
NADH
-
45°C, pH 6.1, cosubstrate: 3-hydroxy-2-butanone, wild-type enzyme
0.38
NADH
mutant enzyme W95L, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
1.1
NADH
-
45°C, pH 6.1, cosubstrate: 3-hydroxy-2-butanone, mutant enzyme K249G/H255R
2
NADH
-
carboxymethylated enzyme, at 55°C
4.5
NADH
-
in 50 mM Tris-HCl (pH 7.5) containing 0.25 mM benzaldehyde, at 65°C
4.5
NADH
-
native enzyme, at 55°C
8.88
NADH
-
enzyme form ADH-3
10.3
NADH
-
wild type enzyme, at 65°C, in the presence of benzaldehyde
10.3
NADH
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
15.6
NADH
cosubstrate acetoin, pH 6.0, 70°C
17.3
NADH
cosubstrate acetoin, pH 6.0, 70°C
25.3
NADH
-
mutant enzyme N249Y, at 65°C, in the presence of benzaldehyde
34.8
NADH
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
52.4
NADH
-
pH 10.5, 65°C
153
NADH
cosubstrate butanal, pH 7.0, 30°C
326
NADH
wild-type, pH 8.0, 60°C
1645.7
NADH
cosubstrate acetaldehyde, pH 8, 60°C
0.03
NADP+
-
45°C, pH 8.8, cosubstrate: 2,3-butanediol, wild-type enzyme
0.073
NADP+
-
pH 8.8, 45°C, wild-type enzyme
0.29
NADP+
-
pH 8.8, 45°C, mutant enzyme G211S
0.52
NADP+
-
pH 8.8, 45°C, mutant enzyme G211InsG
0.86
NADP+
-
pH 8.8, 45°C, mutant enzyme G211InsC
0.92
NADP+
-
pH 8.8, 45°C, mutant enzyme G211C
2.1
NADP+
-
pH 8.8, 45°C, mutant enzyme G211InsA
2.1
NADP+
-
pH 8.8, 45°C, mutant enzyme G211InsS
4.7
NADP+
-
45°C, pH 8.8, cosubstrate: 2,3-butanediol, mutant enzyme K249G/H255R
10.5
NADP+
cosubstrate 1-phenylethanol, pH 9.0, 70°C
30
NADP+
cosubstrate 2,3-butanediol, pH 9.0, 70°C
43.9
NADP+
cosubstrate ethanol, pH 8, 60°C
0.2
NADPH
-
45°C, pH 6.1, cosubstrate: 3-hydroxy-2-butanone, wild-type enzyme
1.2
NADPH
-
45°C, pH 6.1, cosubstrate: 3-hydroxy-2-butanone, mutant enzyme K249G/H255R
3.33
octanal
-
-
17
octanal
-
enzyme form ADH-3
0.038
Octanol
-
recombinant allozyme Ile308, pH 7.5, 25°C
0.04
Octanol
-
recombinant allozyme Val308, pH 7.5, 25°C
3.17
Octanol
pH and temperature not specified in the publication
4.33
Octanol
-
isoenzyme alpha,gamma1
0.245
Pentanol
-
-
0.283
Pentanol
-
pyrazole-sensitive enzyme, pH 7.5
0.333
Pentanol
-
pyrazole-sensitive enzyme, pH 10.0
0.667
Pentanol
-
pH 7.5, anodic enzyme form
18.4
Pentanol
-
mutant enzyme W54L
56.2
Pentanol
-
wild-type enzyme Adh 1
202
phenylacetaldehyde
-
pH 6.0, 37°C, recombinant enzyme expressed from Saccharomyces cerevisiae
264
phenylacetaldehyde
-
pH 6.0, 37°C, recombinant enzyme expressed from Hansenula polymorpha
303
phenylacetaldehyde
-
pH 6.0, 37°C, recombinant enzyme expressed from Arxula adeninivorans
5.8
propan-2-ol
-
mutant enzyme W54L
53.1
propan-2-ol
-
wild-type enzyme Adh 1
17.2
Propanol
-
-
28.7
Propanol
-
mutant enzyme W54L
0.087
retinol
-
recombinant allozyme Ile308, pH 7.5, 25°C
0.11
retinol
-
recombinant allozyme Val308, pH 7.5, 25°C
0.21
trans-cinnamaldehyde
mutant enzyme W95L,, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
2.1
trans-cinnamaldehyde
wild type enzyme, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
18.9
trans-cinnamaldehyde
mutant enzyme W95L/N249Y, in 0.1 M glycine-NaOH buffer (pH 10.5), at 65°C
418
Valeraldehyde
-
pH 6.0, 37°C, recombinant enzyme expressed from Saccharomyces cerevisiae
432
Valeraldehyde
-
pH 6.0, 37°C, recombinant enzyme expressed from Arxula adeninivorans
528
Valeraldehyde
-
pH 6.0, 37°C, recombinant enzyme expressed from Hansenula polymorpha
0.467
Vanillyl alcohol
-
-
8.67
Vanillyl alcohol
-
-
additional information
additional information
-
-
-
additional information
additional information
-
turnover-numbers for the class I isoenzymes with the substrates ethanol, methanol, ethylene glycol, benzyl alcohol, octanol, cyclohexanol and 16-hydroxyhexadecanoic acid
-
additional information
additional information
-
Km-values of active-site Co(II)substituted enzyme
-
additional information
additional information
-
kinetics of ethanol oxidation
-
additional information
additional information
-
kinetics of ethanol oxidation
-
additional information
additional information
-
kcat for isozymes ADH1, and ADH4 for all retinoid substrates in forward and reverse reaction
-
additional information
additional information
-
kcat for isozymes ADH1B1, ADH1B2, and ADH4 for all retinoid substrates in forward and reverse reaction
-
additional information
additional information
-
effects of tert-butanol, butyramide, valeramide and capronamide on turnover-number of ethanol
-
additional information
additional information
-
kinetic data füor wild-type enzyme and chimeric enzyme created by insertion of an RTX domain from the adenylate cyclase of Bordetella pertussis into a loop near the catalytic active site of the thermostable alcohol dehydrogenase D (AdhD) from Pyrococcus furiosus
-