Reaction catalyzed by tRNA (N6-L-threonylcarbamoyladenosine37-C2)-methylthiotransferase (2.8.4.5)

		+		2-sulfanyl-N6-L-threonylcarbamoyladenine37 in tRNA	=			+		2-(methylsulfanyl)-N6-L-threonylcarbamoyladenine37 in tRNA
	S-adenosyl-L-methionine	+		2-sulfanyl-N6-L-threonylcarbamoyladenine37 in tRNA	=		S-adenosyl-L-homocysteine	+		2-(methylsulfanyl)-N6-L-threonylcarbamoyladenine37 in tRNA