Reaction catalyzed by cholest-4-en-3-one 26-monooxygenase [(25S)-3-oxocholest-4-en-26-oate forming] (1.14.15.29)
+
2
reduced ferredoxin [iron-sulfur] cluster
+
2
+
=
+
2
oxidized ferredoxin [iron-sulfur] cluster
+
H2O
cholest-4-en-3-one
+
2 reduced ferredoxin [iron-sulfur] cluster
+
2 H+
+
O2
=
27-hydroxycholest-4-en-3-one
+
2 oxidized ferredoxin [iron-sulfur] cluster
+
H2O