Reaction catalyzed by tRNA (uracil54-C5)-methyltransferase (2.1.1.35)

		+			=			+		5-methyluridine54 in tRNA
	S-adenosyl-L-methionine	+		uridine54 in tRNA	=		S-adenosyl-L-homocysteine	+		5-methyluridine54 in tRNA