Sequence of PNGF_ELIMR
EC Number:3.5.1.52
EC Number
Recommended Name
Accession Code
Organism
No of amino acids
Molecular Weight [Da]
Source
peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase
P21163
Elizabethkingia miricola
354
39032
Reaction
Manalpha(1-6)(Xylbeta(1-2))Manbeta(1-4)GlcNAcbeta(1-4)(Fucalpha(1-3))GlcNAc-Asn-Asp-Glu-Ser-Ser + H2O = Manalpha(1-6)(Xylbeta(1-2))Manbeta(1-4)GlcNAcbeta(1-4)(Fucalpha(1-3))GlcNAc + Asn-Asp-Glu-Ser-Ser
Other sequences found for EC No. 3.5.1.52
General information:
Sequence
0 MRKLLIFSIS AYLMAGIVSC KGVDSATPVT EDRLALNAVN APADNTVNIK TFDKVKNAFG
60 DGLSQSAEGT FTFPADVTTV KTIKMFIKNE CPNKTCDEWD RYANVYVKNK TTGEWYEIGR
120 FITPYWVGTE KLPRGLEIDV TDFKSLLSGN TELKIYTETW LAKGREYSVD FDIVYGTPDY
180 KYSAVVPVIQ YNKSSIDGVP YGKAHTLGLK KNIQLPTNTE KAYLRTTISG WGHAKPYDAG
240 SRGCAEWCFR THTIAINNAN TFQHQLGALG CSANPINNQS PGNWAPDRAG WCPGMAVPTR
300 IDVLNNSLTG STFSYEYKFQ SWTNNGTNGD AFYAISSFVI AKSNTPISAP VVTN
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Sequence Reference
Authors
Title
Journal
Volume
Pages
Year
PubMed ID
335401
Tarentino A.L.,Quinones G.,Trumble A.,Changchien L.-M.,Duceman B.,Maley F.,Plummer T.H. Jr.
Molecular cloning and amino acid sequence of peptide-N4-(N-acetyl-beta-D-glucosaminyl)asparagine amidase from flavobacterium meningosepticum.
J. Biol. Chem.
265
6961-6966
1990
335402
Barsomian G.D.,Johnson T.L.,Borowski M.,Denman J.,Ollington J.F.,Hirani S.,McNeilly D.S.,Rasmussen J.R.
Cloning and expression of peptide-N4-(N-acetyl-beta-D-glucosaminyl)asparagine amidase F in Escherichia coli.
J. Biol. Chem.
265
6967-6972
1990
335403
Lemp D.,Haselbeck A.,Klebl F.
Molecular cloning and heterologous expression of N-glycosidase F from Flavobacterium meningosepticum.
J. Biol. Chem.
265
15606-15610
1990
335404
Kuhn P.,Tarantino A.L.,Plummer T.H. Jr.,van Roey P.
Crystal structure of peptide-N4-(N-acetyl-beta-D-glucosaminyl)asparagine amidase F at 2.2-A resolution.
Biochemistry
33
11699-11706
1994
335405
Norris G.E.,Stillman T.J.,Anderson B.F.,Baker E.N.
The three-dimensional structure of PNGase F, a glycosylasparaginase from Flavobacterium meningosepticum.
Structure
2
1049-1059
1994
335406
Kuhn P.,Guan C.,Cui T.,Tarentino A.L.,Plummer T.H. Jr.,van Roey P.
Active site and oligosaccharide recognition residues of peptide-N4-(N-acetyl-beta-D-glucosaminyl)asparagine amidase F.
J. Biol. Chem.
270
29493-29497
1995
