Sequence of AMYS_NEIPO
EC Number:2.4.1.4
EC Number
Recommended Name
Accession Code
Organism
No of amino acids
Molecular Weight [Da]
Source
Reaction
sucrose + [(1->4)-alpha-D-glucosyl]n = D-fructose + [(1->4)-alpha-D-glucosyl]n+1
Other sequences found for EC No. 2.4.1.4
General information:
Sequence
0 MLTPTQQVGL ILQYLKTRIL DIYTPEQRAG IEKSEDWRQF SRRMDTHFPK LMNELDSVYG
60 NNEALLPMLE MLLAQAWQSY SQRNSSLKDI DIARENNPDW ILSNKQVGGV CYVDLFAGDL
120 KGLKDKIPYF QELGLTYLHL MPLFKCPEGK SDGGYAVSSY RDVNPALGTI GDLREVIAAL
180 HEAGISAVVD FIFNHTSNEH EWAQRCAAGD PLFDNFYYIF PDRRMPDQYD RTLREIFPDQ
240 HPGGFSQLED GRWVWTTFNS FQWDLNYSNP WVFRAMAGEM LFLANLGVDI LRMDAVAFIW
300 KQMGTSCENL PQAHALIRAF NAVMRIAAPA VFFKSEAIVH PDQVVQYIGQ DECQIGYNPL
360 QMALLWNTLA TREVNLLHQA LTYRHNLPEH TAWVNYVRSH DDIGWTFADE DAAYLGISGY
420 DHRQFLNRFF VNRFDGSFAR GVPFQYNPST GDCRVSGTAA ALVGLAQDDP HAVDRIKLLY
480 SIALSTGGLP LIYLGDEVGT LNDDDWSQDS NKSDDSRWAH RPRYNEALYA QRNDPSTAAG
540 QIYQGLRHMI AVRQSNPRFD GGRLVTFNTN NKHIIGYIRN NALLAFGNFS EYPQTVTAHT
600 LQAMPFKAHD LIGGKTVSLN QDLTLQPYQV MWLEIA
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Sequence Reference
Authors
Title
Journal
Volume
Pages
Year
PubMed ID
513819
Buettcher V.,Welsh T.,Willmitzer L.,Kossmann J.
Cloning and characterization of the gene for amylosucrase from Neisseria polysaccharea: production of a linear alpha-1,4-glucan.
J. Bacteriol.
179
3324-3330
1997
513820
Potocki de Montalk G.,Remaud-Simeon M.,Willemot R.-M.,Planchot V.,Monsan P.
Sequence analysis of the gene encoding amylosucrase from Neisseria polysaccharea and characterization of the recombinant enzyme.
J. Bacteriol.
181
375-381
1999
513821
Zhu P.,Tsang R.S.W.,Tsai C.-M.
Nonencapsulated Neisseria meningitidis strain produces amylopectin from sucrose: altering the concept for differentiation between N. meningitidis and N. polysaccharea.
J. Clin. Microbiol.
41
273-278
2003
513822
Potocki de Montalk G.,Remaud-Simeon M.,Willemot R.-M.,Sarcabal P.,Planchot V.,Monsan P.
Amylosucrase from Neisseria polysaccharea: novel catalytic properties.
FEBS Lett.
471
219-223
2000
513823
Sarcabal P.,Remaud-Simeon M.,Willemot R.-M.,Potocki de Montalk G.,Svensson B.,Monsan P.
Identification of key amino acid residues in Neisseria polysaccharea amylosucrase.
FEBS Lett.
474
33-37
2000
513824
Mirza O.,Skov L.K.,Remaud-Simeon M.,Potocki de Montalk G.,Albenne C.,Monsan P.,Gajhede M.
Crystal structures of amylosucrase from Neisseria polysaccharea in complex with D-glucose and the active site mutant Glu328Gln in complex with the natural substrate sucrose.
Biochemistry
40
9032-9039
2001
513825
Skov L.K.,Mirza O.,Henriksen A.,De Montalk G.P.,Remaud-Simeon M.,Sarcabal P.,Willemot R.M.,Monsan P.,Gajhede M.
Amylosucrase, a glucan-synthesizing enzyme from the alpha-amylase family.
J. Biol. Chem.
276
25273-25278
2001
513826
Skov L.K.,Mirza O.,Sprogoe D.,Dar I.,Remaud-Simeon M.,Albenne C.,Monsan P.,Gajhede M.
Oligosaccharide and sucrose complexes of amylosucrase. Structural implications for the polymerase activity.
J. Biol. Chem.
277
47741-47747
2002
513827
Jensen M.H.,Mirza O.,Albenne C.,Remaud-Simeon M.,Monsan P.,Gajhede M.,Skov L.K.
Crystal structure of the covalent intermediate of amylosucrase from Neisseria polysaccharea.
Biochemistry
43
3104-3110
2004
