Sequence of TGT_ECOLI
EC Number:2.4.2.29
EC Number
Recommended Name
Accession Code
Organism
No of amino acids
Molecular Weight [Da]
Source
Reaction
guanine34 in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine34 in tRNA + guanine
Other sequences found for EC No. 2.4.2.29
General information:
Sequence
0 MKFELDTTDG RARRGRLVFD RGVVETPCFM PVGTYGTVKG MTPEEVEATG AQIILGNTFH
60 LWLRPGQEIM KLHGDLHDFM QWKGPILTDS GGFQVFSLGD IRKITEQGVH FRNPINGDPI
120 FLDPEKSMEI QYDLGSDIVM IFDECTPYPA DWDYAKRSME MSLRWAKRSR ERFDSLGNKN
180 ALFGIIQGSV YEDLRDISVK GLVDIGFDGY AVGGLAVGEP KADMHRILEH VCPQIPADKP
240 RYLMGVGKPE DLVEGVRRGI DMFDCVMPTR NARNGHLFVT DGVVKIRNAK YKSDTGPLDP
300 ECDCYTCRNY SRAYLHHLDR CNEILGARLN TIHNLRYYQR LMAGLRKAIE EGKLESFVTD
360 FYQRQGREVP PLNVD
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Sequence Reference
Authors
Title
Journal
Volume
Pages
Year
PubMed ID
108403
Reuter K.,Slany R.,Ullrich F.,Kersten H.
Structure and organization of Escherichia coli genes involved in biosynthesis of the deazaguanine derivative queuine, a nutrient factor for eukaryotes.
J. Bacteriol.
173
2256-2264
1991
108404
Garcia G.A.,Koch K.A.,Chong S.
tRNA-guanine transglycosylase from Escherichia coli. Overexpression, purification and quaternary structure.
J. Mol. Biol.
231
489-497
1993
108406
Blattner F.R.,Plunkett G. III,Bloch C.A.,Perna N.T.,Burland V.,Riley M.,Collado-Vides J.,Glasner J.D.,Rode C.K.,Mayhew G.F.,Gregor J.,Davis N.W.,Kirkpatrick H.A.,Goeden M.A.,Rose D.J.,Mau B.,Shao Y.
The complete genome sequence of Escherichia coli K-12.
Science
277
1453-1462
1997
108407
Hayashi K.,Morooka N.,Yamamoto Y.,Fujita K.,Isono K.,Choi S.,Ohtsubo E.,Baba T.,Wanner B.L.,Mori H.,Horiuchi T.
Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.
Mol. Syst. Biol.
2
0-0
2006
108408
Gardel C.,Johnson K.,Jacq A.,Beckwith J.
The secD locus of E.coli codes for two membrane proteins required for protein export.
EMBO J.
9
3209-3216
1990
108410
Pogliano K.J.,Beckwith J.
Genetic and molecular characterization of the Escherichia coli secD operon and its products.
J. Bacteriol.
176
804-814
1994
108411
Reuter K.,Chong S.,Ullrich F.,Kersten H.,Garcia G.A.
Serine 90 is required for enzymic activity by tRNA-guanine transglycosylase from Escherichia coli.
Biochemistry
33
7041-7046
1994
108412
Chong S.,Curnow A.W.,Huston T.J.,Garcia G.A.
tRNA-guanine transglycosylase from Escherichia coli is a zinc metalloprotein. Site-directed mutagenesis studies to identify the zinc ligands.
Biochemistry
34
3694-3701
1995
108413
Garcia G.A.,Chong S.
Cysteine 265 is in the active site of, but is not essential for catalysis by tRNA-guanine transglycosylase (TGT) from Escherichia coli.
J. Protein Chem.
16
11-17
1997
108414
Kittendorf J.D.,Barcomb L.M.,Nonekowski S.T.,Garcia G.A.
tRNA-guanine transglycosylase from Escherichia coli: molecular mechanism and role of aspartate 89.
Biochemistry
40
14123-14133
2001
108415
Kittendorf J.D.,Sgraja T.,Reuter K.,Klebe G.,Garcia G.A.
An essential role for aspartate 264 in catalysis by tRNA-guanine transglycosylase from Escherichia coli.
J. Biol. Chem.
278
42369-42376
2003
