EC Number   |
Protein Variants |
Reference |
|---|
   6.5.1.1 | A287K |
the mutant enzyme has a half-life time at 94°C of 20 min |
747085 |
| 6.5.1.1 | A387K |
the mutant enzyme has a half-life time at 94°C of 15 min |
747085 |
| 6.5.1.1 | A576P |
the mutation hardly affects ligation activity at pH 3.0 |
-, 694908 |
| 6.5.1.1 | Cdel5 |
mutant lacking the C-terminal pentapeptide HEEDR of motif VI, 1% of wild-type DNA ligase activity |
653371 |
| 6.5.1.1 | CDELTA1 |
mutant with deleted C-terminal five amino acids: mutant shows 34% the specific activity of wild-type. Preformed Lig-AMP in vivo: 71% of total enzyme (wild-type: 70%), preformed Lig-AMP in vitro: 8% of total enzyme (wild-type: 21%) |
727917 |
| 6.5.1.1 | CDELTA2 |
mutant with deleted C-terminal five amino acids: mutant shows 6% the specific activity of wild-type. Preformed Lig-AMP in vivo: 80% of total enzyme (wild-type: 70%), preformed Lig-AMP in vitro: 1.1% of total enzyme (wild-type: 21%) |
727917 |
| 6.5.1.1 | CDELTA3 |
mutant with deleted C-terminal five amino acids: mutant shows 2% the specific activity of wild-type. Preformed Lig-AMP in vivo: 67% of total enzyme (wild-type: 70%), preformed Lig-AMP in vitro: 1.3% of total enzyme (wild-type: 21%) |
727917 |
| 6.5.1.1 | CDELTA4 |
mutant with deleted C-terminal five amino acids: mutant shows 1% the specific activity of wild-type. Preformed Lig-AMP in vivo: 18% of total enzyme (wild-type: 70%), preformed Lig-AMP in vitro: 0.8% of total enzyme (wild-type: 21%) |
727917 |
| 6.5.1.1 | CDELTA5 |
mutant with deleted C-terminal five amino acids: mutant shows 1% the specific activity of wild-type and is poorly responsive to ATP. Preformed Lig-AMP in vivo: 7% of total enzyme (wild-type: 70%), preformed Lig-AMP in vitro: 0.5% of total enzyme (wild-type: 21%) |
727917 |
| 6.5.1.1 | D15A |
3'-ribonuclease activity with D10R2 primer-template is 110% of wild-type activity, 3'-phosphomonoesterase activity with D11-p primer-template is 3.3% of wild-type activity, 3'-phosphomonoesterase activity with D9R1-p primer template is 4.9% of wild-type activity |
662411 |
