   6.2.1.67 | more |
gene disruption mutagenesis in phsC using the temperature-sensitive plasmid pDS104 is performed. Mutants PHSB and PHSBC are generated using the nonreplicative plasmids pMS100 and pDS199, respectively, complementation of the mutants PHSB and PHSBC and heterologous expression of phsB/phsC in Streptomyces lividans. Construction of a phsB null mutant (B3-14), which is unable to produce phosphinothricin tripeptide (PTT), no alanine-activating activity attributable to PTT synthetase III is detected. Construction of a triple PTT mutant (phsB, orfM, and phsC), mutant PHSBC has lost the ability to produce PTT. Transformation of PHSBC with either phsB including orfM (pDS207) or phsC (pDS208) does not restore PTT synthesis, indicating that PhsB cannot take on the function of PhsC or vice versa |
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| 6.2.1.67 | more |
gene disruption mutagenesis in phsC using the temperature-sensitive plasmid pDS104 is performed. Mutants PHSB and PHSBC are generated using the nonreplicative plasmids pMS100 and pDS199, respectively, complementation of the mutants PHSB and PHSBC and heterologous expression of phsB/phsC in Streptomyces lividans. No activity attributable to PTT synthetase II or phosphinothricin tripeptide (PTT) synthesis is detected in the phsC disruption mutant. Construction of a triple PTT mutant (phsB, orfM, and phsC), mutant PHSBC has lost the ability to produce PTT. Transformation of PHSBC with either phsB including orfM (pDS207) or phsC (pDS208) does not restore PTT synthesis, indicating that PhsB cannot take on the function of PhsC or vice versa |
-, 760352 |
