EC Number |
Protein Variants |
Reference |
---|
6.1.1.15 | A57G |
site-directed mutagenesis, the A57G mutation introduces a BstBI restriction site within the gene, but has no effect on catalytic activity |
694434 |
6.1.1.15 | C443A |
mutagenesis of C443 to amino acids Ala, Gly and Ser results in significant decreases, 16fold to 225fold in kcat/KmPro, as measured by the ATP-diphosphate exchange assay. The Ala and Gly mutations have relatively small effect, 4fold to 7fold, on the overall aminoacylation reaction, while the activity of the C443 mutant in this same assay is substantially reduced, 80fold |
164 |
6.1.1.15 | C443G |
mutagenesis of C443 to amino acids Ala, Gly and Ser results in significant decreases, 16fold to 225fold in kcat/KmPro, as measured by the ATP-diphosphate exchange assay. The Ala and Gly mutations have relatively small effect, 4fold to 7fold, on the overall aminoacylation reaction, while the activity of the C443 mutant in this same assay is substantially reduced, 80fold |
164 |
6.1.1.15 | C443S |
mutagenesis of C443 to amino acids Ala, Gly and Ser results in significant decreases, 16fold to 225fold in kcat/KmPro, as measured by the ATP-diphosphate exchange assay. The Ala and Gly mutations have relatively small effect, 4fold to 7fold, on the overall aminoacylation reaction, while the activity of the C443 mutant in this same assay is substantially reduced, 80fold |
164 |
6.1.1.15 | D198A |
the overall aminoacylation activity of the mutant is reduced 5.5fold |
727022 |
6.1.1.15 | D350A |
site-directed mutagenesis, subdomain III mutant, residual remaining aminoaclyation activity, no pre-transfer editing activity |
650167 |
6.1.1.15 | D378A |
site-directed mutagenesis, subdomain III mutant, reduced aminoaclyation and pre-transfer editing activity |
650167 |
6.1.1.15 | D386A |
site-directed mutagenesis, subdomain III mutant, reduced aminoaclyation and pre-transfer editing activity |
650167 |
6.1.1.15 | D394A |
site-directed mutagenesis, subdomain III mutant, reduced aminoaclyation and pre-transfer editing activity |
650167 |
6.1.1.15 | E103A |
abolished activity with L-proline |
651296 |