EC Number |
Protein Variants |
Reference |
---|
5.1.3.2 | A25V |
the mutant shows reduced activity compared to the wild-type enzyme |
703639 |
5.1.3.2 | C300Y |
site-directed mutagenesis, the mutation results in decreased activity toward UDP-GlcNAc and UDP-GalNAc |
-, 747049 |
5.1.3.2 | C307Y |
normal activity with respect to UDP-galactose, complete loss of activity with respect to UDP-N-acetylgalactosamine |
662246 |
5.1.3.2 | D103G |
the mutant demonstrates 82.1% residual activity |
714374 |
5.1.3.2 | D103G |
the ratio of turnover number to Km-value is 14.4fold lower than the wild-type ratio |
661710 |
5.1.3.2 | D69E |
the mutant shows reduced activity compared to the wild-type enzyme |
703639 |
5.1.3.2 | E165K |
unstable mutant |
703639 |
5.1.3.2 | G118A/G119A |
site-directed mutagenesis, the mutant shows highly reduced activity with UDP-N-acetylglucosamine and reduced activity with UDP-Gal, the mutant's substrate specificity is shifted toward non-acetylated substrates |
726782 |
5.1.3.2 | G188S/G119S |
site-directed mutagenesis, the mutant shows highly reduced activity with UDP-N-acetylglucosamine and reduced activity with UDP-Gal, the mutant's substrate specificity is shifted toward non-acetylated substrates |
726782 |
5.1.3.2 | G302D |
the mutant enzyme is not able to rescue galactose-sensitive cell proliferation when stably expressed in ldlD cells |
703639 |