EC Number   |
Protein Variants |
Reference |
|---|
    4.2.1.104 | E107A |
site-directed mutagenesis, soluble, no activity |
692457 |
| 4.2.1.104 | E107D |
site-directed mutagenesis, soluble, no activity |
692457 |
| 4.2.1.104 | E94L |
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme. The mutant can form trimers or a mixture of polymers but not decamers |
-, 716700 |
| 4.2.1.104 | more |
a delta cyn1 knockout in Sordaria macrospora is totally devoid of cyanase activity and shows an increased sensitivity to exogenously supplied cyanate in an arginine-depleted medium, defects in ascospore germination, but no other obvious morphological phenotype |
692457 |
| 4.2.1.104 | more |
Arg104, Glu107 and Ser130 are essential for enzyme activity of the fungal cyanase, irrespectively of substitution by Alanine or another amino-acid |
692457 |
| 4.2.1.104 | more |
construction of CYN knockout plants |
-, 716700 |
| 4.2.1.104 | more |
mutant lacking the cynS gene, mutant is unable to use cyanate as the sole nitrogen source but shows the same resistance to cyanate as the wild-type strain, cynS-mutant is not affected in its ability to degrade cyanide, which unambiguously indicates that cyanate is not a central metabolite in cyanide assimilation |
-, 690549 |
| 4.2.1.104 | more |
targeted mutants of Synechococcus elongatus strain PCC7942 disrupted in the coding sequences of the gene encoding the cytosoliyc cyanase (CynS) to study metabolism of exogenously supplied cyanate |
-, 680479 |
| 4.2.1.104 | more |
targeted mutants of Synechococcus elongatus strain PCC7942 impaired in the CO2-concentrating mechanism CCM to study metabolism of exogenously supplied cyanate in cyanobacteria, inactivation of CCM impairs metabolism of external cyanate by insufficient internal pools of HCO3- and CO2 resulting in low levels of the co-substrate bicarbonate that is required for cyanase activity |
-, 680479 |
| 4.2.1.104 | more |
targeted mutants of Synechococcus elongatus strain PCC7942, disruption of the cynA gene, encoding a binding protein of a multicomponent ABC-transporter, inactivation of CynA does not affect cyanase function but inhibits decomposition of external cyanate |
-, 680479 |
