EC Number   |
Protein Variants |
Reference |
|---|
   3.4.24.27 | A113D |
complete loss of activity |
698656 |
| 3.4.24.27 | A113E |
complete loss of activity |
698656 |
| 3.4.24.27 | A113H |
complete loss of activity |
698656 |
| 3.4.24.27 | A113K |
complete loss of activity |
698656 |
| 3.4.24.27 | A113R |
complete loss of activity |
698656 |
| 3.4.24.27 | A4T/G8C/T56A/G58A/N60C/T63F/S65P/A69P |
the mutant shows altered thermodynamics |
683276 |
| 3.4.24.27 | A4T/T56A/G58A/T63F/S65P/A69P |
the mutant shows altered thermodynamics |
683276 |
| 3.4.24.27 | D150A |
131% residual activity with casein, 81% residual activity with substrate N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide |
698656 |
| 3.4.24.27 | D150A |
mutant with improved activity, the mutant has higher kcat values in N-carbobenzoxy-L-Asp-L-Phe-methyl ester synthesis than wild type |
711866 |
| 3.4.24.27 | D150E |
128% residual activity with casein, 228% residual activity with substrate N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide |
698656 |
