EC Number |
Protein Variants |
Reference |
---|
3.4.11.9 | D260A |
MnA is bound at the active site, but the MnB site is empty. Loss of catalytic activity |
667743 |
3.4.11.9 | D260A |
no enzymic activity |
667743 |
3.4.11.9 | D271A |
no atoms of Mn(II) are bound at the active site. Loss of catalytic activity |
667743 |
3.4.11.9 | D271A |
no enzymic activity |
667743 |
3.4.11.9 | D449A |
site-directed mutagenesis, inactive mutant |
649942 |
3.4.11.9 | D449N |
site-directed mutagenesis, inactive mutant |
649942 |
3.4.11.9 | D460A |
site-directed mutagenesis, inactive mutant |
649942 |
3.4.11.9 | D460N |
site-directed mutagenesis, inactive mutant |
649942 |
3.4.11.9 | D53A |
site-directed mutagenesis, mutant D53A is significantly less active than the wild-type protein toward Xaa-Pro-Ala tripeptides |
755354 |
3.4.11.9 | E383A |
both Mn-(II) atoms are present in the active site, but the bridging solvent molecule is located significantly farther from the metals than in wild-type. Loss of catalytic activity |
667743 |