EC Number |
Protein Variants |
Reference |
---|
3.2.1.40 | D330A |
2350fold reduction in specific activity as compared to the wild-type enzyme |
-, 749441 |
3.2.1.40 | D335A |
560fold reduction in specific activity as compared to the wild-type enzyme |
-, 749441 |
3.2.1.40 | D335E |
2770fold reduction in specific activity as compared to the wild-type enzyme |
749441 |
3.2.1.40 | D335N |
1170fold reduction in specific activity as compared to the wild-type enzyme |
-, 749441 |
3.2.1.40 | D342A |
7670fold reduction in specific activity as compared to the wild-type enzyme |
-, 749441 |
3.2.1.40 | D503A |
no detectable activity |
749745 |
3.2.1.40 | D552A |
kcat/KM value is 65% of the wild-type value. This reduced catalytic efficiency is mainly due to a significative variation in the turnover number, defined by the kcat value, for which a 34% decrease is observed |
749745 |
3.2.1.40 | D552A |
mutant enzyme shows 47.9% of residual activity |
749745 |
3.2.1.40 | D567N |
mutagenesis of catalytic amino acid residues identifed by crystallization studies, enzyme activity reduced |
-, 681462 |
3.2.1.40 | D579N |
mutagenesis of catalytic amino acid residues identifed by crystallization studies, enzyme activity reduced |
-, 681462 |