EC Number   |
Protein Variants |
Reference |
|---|
    3.1.1.79 | A152F |
site-directed mutagenesis, the mutant shows 12.83% activity compared to wild-type |
750177 |
| 3.1.1.79 | C101S |
site-directed mutagenesis, the mutant shows 31.55% activity compared to wild-type |
-, 750177 |
| 3.1.1.79 | C103S |
site-directed mutagenesis, the mutant shows 17.95% activity compared to wild-type |
-, 750177 |
| 3.1.1.79 | C184S |
site-directed mutagenesis, the mutant shows 16.93% activity compared to wild-type |
750177 |
| 3.1.1.79 | C94S |
site-directed mutagenesis, the mutant shows 11.90% activity compared to wild-type |
-, 750177 |
| 3.1.1.79 | D244N |
site-directed mutagenesis, the mutant shows 1.55% activity compared to wild-type |
750177 |
| 3.1.1.79 | D287A |
active site mutant, significant decrease in activity, mutant forms oligomers instead of dimers |
752198 |
| 3.1.1.79 | DELTA644-658 |
reduced basal hydrolytic activity, no activation by cyclic AMP-dependent protein kinase |
664085 |
| 3.1.1.79 | DELTA657-664 |
reduced basal hydrolytic activity, no activation by cyclic AMP-dependent protein kinase |
664085 |
| 3.1.1.79 | DELTA666-683 |
very low basal and stimulated hydrolytic activity. Vmax with cholesteryl ester is 29fold lower than wild-type value. Vmax with p-nitrophenylbutyrate is 8fold lower than wild-type value |
664085 |
