EC Number   |
Protein Variants |
Reference |
|---|
   3.1.1.25 | C284A |
no lactonase activity with either homogentisic acid lactone or undecanoic-delta-lactone |
650953 |
| 3.1.1.25 | D183N |
the mutant disfavors paraoxon binding due to its charged nature and possible electrostatic repulsion with the phosphate group of paraoxon |
695051 |
| 3.1.1.25 | E14K |
Tm-value for the mutant enzyme is 6°C lower than wild-type Tm-value. The efficiency of methyl-paraoxon hydrolysis is similar for wild-type and the mutants, whereas the mutants exhibit higher catalytic efficiency against ethyl-paraoxon than the wild-type. The mutants exhibit a heavily reduced N-acyl-homoserine lactonase-, delta-lactonase- and gamma-lactonase activity |
-, 726306 |
| 3.1.1.25 | E14K/Y34Q |
Tm-value for the mutant enzyme is 4°C lower than wild-type Tm-value. The efficiency of methyl-paraoxon hydrolysis is similar for wild-type and the mutants, whereas the mutants exhibit higher catalytic efficiency against ethyl-paraoxon than the wild-type. The mutants exhibit a heavily reduced N-acyl-homoserine lactonase-, delta-lactonase- and gamma-lactonase activity |
726306 |
| 3.1.1.25 | F222D |
the mutation abolishes both esterase and PON activity of PON1 |
695051 |
| 3.1.1.25 | F222Y |
the mutation abolishes the PON activity of PON1 but retains the esterase activity with a 1.5fold increase in KM for phenyl acetate |
695051 |
| 3.1.1.25 | H115A |
activity with 5-(thioethyl)butyrolactone is 1.1% of wild-type activity, activity with 5-(thiobutyl)butyrolactone is 4.2% of wild-type activity, activity with delta-valerolactone, gamma-nonalactone or gamma-undecanoic lactone is less than 1% of wild-type activity |
665745 |
| 3.1.1.25 | H115A |
activity with dicoumarin is 366% compared to wild-type activity, activity with delta-valerolactone is 3% of wild-type activity, activity with gamma-nonalactone is 1% of wild-type activity |
665745 |
| 3.1.1.25 | H115Q |
activity with dicoumarin is 268% compared to wild-type activity, activity with delta-valerolactone is 4.96% of wild-type activity, activity with gamma-nonalactone is 1.2% of wild-type activity. kcat/Km for delta-valerolactone is 2.2% of wild-type value, kcat/Km for gamma-nonalactone is 0.3% of wild-type value, kcat/Km for gamma-caprolactone is 1.4% of wild-type value, kcat/Km for 5-(thiobutyl)butyrolactone is 1.1% of wild-type value. Mutant with reduced lactonase activity, exhibits reduced or no biological function in ex vivo assay |
665745 |
| 3.1.1.25 | H115Q/H134Q |
activity with 5-(thioethyl)butyrolactone is 0.2% compared to wild-type activity, activity with 5-(thiobutyl)butyrolactone is 0.6% of wild-type activity, activity with delta-valerolactone, gamma-nonalactone or gamma-undecanoic lactone is less than 1% of wild-type activity |
665745 |
