EC Number   |
Protein Variants |
Reference |
|---|
    2.5.1.78 | A56S |
kcat is 81.3% of wild-type value |
699514 |
| 2.5.1.78 | D138A |
kcat is 91.5% of wild-type value |
699514 |
| 2.5.1.78 | D44G/C93S/C139S/T118A |
mutant constructed to improve the overexpression and purification of the molecule as well as to obtain new crystal forms. Two cysteines are replaced to bypass misfolding problems and a charged surface residue is replaced to force different molecular packings. Mutant crystallizes in space group R3 and diffracts to 1.6 A resolution |
684198 |
| 2.5.1.78 | E58Q |
kcat is 70.2% of wild-type value |
699514 |
| 2.5.1.78 | F113S |
kcat is 6.8% of wild-type value |
699514 |
| 2.5.1.78 | F22D |
kcat is 14.5% of wild-type value |
699514 |
| 2.5.1.78 | F22S |
kcat is 47.2% of wild-type value |
699514 |
| 2.5.1.78 | F22V |
kcat is 26.4% of wild-type value |
699514 |
| 2.5.1.78 | F22W |
kcat is 43.8% of wild-type value |
699514 |
| 2.5.1.78 | F57S |
kcat is 43.8% of wild-type value |
699514 |
