EC Number   |
Protein Variants |
Reference |
|---|
   2.3.2.10 | D109N |
site-directed mutagenesis, loss of 99% activity compared to the wild-type |
487793 |
| 2.3.2.10 | E205Q |
site-directed mutagenesis, unaltered activity compared to the wild-type |
487793 |
| 2.3.2.10 | E215Q |
site-directed mutagenesis, loss of 15% activity compared to the wild-type |
487793 |
| 2.3.2.10 | E316Q |
site-directed mutagenesis, loss of 60% activity compared to the wild-type |
487793 |
| 2.3.2.10 | E320Q |
site-directed mutagenesis, loss of 96% activity compared to the wild-type |
487793 |
| 2.3.2.10 | F70L |
35% loss of activity |
-, 659068 |
| 2.3.2.10 | K36M |
activity complete abolished |
-, 659068 |
| 2.3.2.10 | K36R |
160-fold reduction of activity, 5.4-fold increased Km for UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine |
659068 |
| 2.3.2.10 | R211K |
17000-fold reduction of activity, no effect on Km for Ala-tRNA, Km for UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine higher than the highest sample concentration |
-, 659068 |
| 2.3.2.10 | Y103F |
no effect on activity |
-, 659068 |
