EC Number |
Protein Variants |
Reference |
---|
2.1.1.204 | C292A |
about 3fold reduction in RNA binding affinity. Significant residual activity |
710547 |
2.1.1.204 | C79A |
about 3fold reduction in RNA binding affinity. No detectable in vitro methylation activity |
710547 |
2.1.1.204 | C79A |
site-directed mutagenesis, inactive mutant |
718931 |
2.1.1.204 | D217H |
site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme |
735726 |
2.1.1.204 | D226Y |
site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme |
735726 |
2.1.1.204 | D255Y |
site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme |
735726 |
2.1.1.204 | E119A |
mutant binds stronger to RNA than wild-type. No detectable in vitro methylation activity |
710547 |
2.1.1.204 | E119A |
site-directed mutagenesis, inactive mutant |
718931 |
2.1.1.204 | E185K |
site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme |
735726 |
2.1.1.204 | E202Q |
site-directed mutagenesis, the mutant shows activity similar to the wild-type enzyme |
735726 |