EC Number   |
Protein Variants |
Reference |
|---|
    1.14.19.1 | C222S |
with Myc epitope tagged to the C terminus, expressed in HeLa cells, actitivity comparable to that of the wild-type |
674542 |
| 1.14.19.1 | C233S |
with Myc epitope tagged to the C terminus, expressed in HeLa cells, actitivity comparable to that of the wild-type |
674542 |
| 1.14.19.1 | C322S |
with Myc epitope tagged to the C terminus, expressed in HeLa cells, actitivity comparable to that of the wild-type |
674542 |
| 1.14.19.1 | C92S |
with Myc epitope tagged to the C terminus, expressed in HeLa cells, actitivity comparable to that of the wild-type |
674542 |
| 1.14.19.1 | C92S/C97S/C222S/C322S |
with Myc epitope tagged to the C terminus, expressed in HeLa cells, actitivity 5fold increased compared with cells transfected with the empty vector, clear ER pattern |
674542 |
| 1.14.19.1 | C97S |
with Myc epitope tagged to the C terminus, expressed in HeLa cells, actitivity comparable to that of the wild-type |
674542 |
| 1.14.19.1 | I112A/E113L |
site-directed mutagenesis, the mutation is able to convert isozyme SCD3 from exclusively a 16:0 desaturase into a predominantly 18:0 desaturase |
745883 |
| 1.14.19.1 | L146F |
point mutation in ssi2 gene, causes reduced desaturase activity |
437672 |
| 1.14.19.1 | more |
activities of enzymes with HA epitope tagged to the N terminus and Myc epitope tagged to the C terminus expressed in HeLa cells are 23fold and 39fold higher than in cells transfected with an empty vector, acitivities of enzymes with HA epitope after amino acid 93 and 138 additionally Myc epitope tagged to the C terminus are 5.5fold and 5.9fold higher than in cells transfected with an empty vector, respectively |
674542 |
| 1.14.19.1 | more |
fusion protein of enzyme isoform ADS1 or ADS2 with plastid targeting sequence of palmitic acid DELTA7 desaturase targets enzymes to plastid and shifts regiospecificity of enzymes from DELTA9 desaturase to DELTA7 desaturase |
660359 |
