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EC Number
Protein Variants
Commentary
Reference
1.14.13.225
E213G
site-directed mutagenesis in the FMO domain
764944
1.14.13.225
E213G/R530G
site-directed mutagenesis
764944
1.14.13.225
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construction of enzyme mutants hMCAL3FMOCH, hMICAL3FMO and hMICAL3FMOCHDELTA213,530. The truncated form containing the FMO and CH domains is much more soluble than the full-length form but still retains catalytic activity for F-actin disassembly
764944
1.14.13.225
more
expression of truncated forms lacking the C-terminal, LIM and/or calponin homology CH regions. The CH, LIM and C-terminal regions lead to an increase of KmNADPH. The C-terminus also determines an about 10fold decrease of kcat
737558
1.14.13.225
R530G
site-directed mutagenesis in the CH domain
764944
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