EC Number   |
Protein Variants |
Reference |
|---|
   1.1.3.4 | A173T/A332S |
increased electron transfer (1.2fold) |
762774 |
| 1.1.3.4 | A173T/F414L |
increased electron transfer (1.2fold), 70% decrease in O2 sensitivity |
762774 |
| 1.1.3.4 | A173V/A332S/F414I/V560T |
increased electron transfer (6.4fold), decrease in O2 sensitivity |
762774 |
| 1.1.3.4 | A332S/V560T |
increased electron transfer (1.2fold), 70% decrease in O2 sensitivity |
762774 |
| 1.1.3.4 | A449C |
site-directed mutagenesis, the mutation results in almost completely diminished activity compared to the wild-type enzyme |
725199 |
| 1.1.3.4 | E84C |
site-directed mutagenesis, the mutation does not affect enzyme activity. Attachment of gold nanoparticles to the purified proteins leads to an immediate and dramatic decrease in activity |
725199 |
| 1.1.3.4 | F414Y |
increased electron transfer |
762774 |
| 1.1.3.4 | F418A |
12.6fold increase in apparent Km value |
-, 726896 |
| 1.1.3.4 | G423D |
site-directed mutagenesis, the mutant shows no activity compared to the wild-type enzyme |
742360 |
| 1.1.3.4 | G423D |
site-directed mutagenesis, the mutants containing the mutation G423D leads to quadruple mutants that are not able to reconstitute. The mutant enzymes displays a dramatic decrease in activity compared to thré wild-type enzyme |
742360 |
