EC Number   |
|---|
   7.4.2.8 | - |
| 7.4.2.8 | 21 kDa N-terminal domain of enzyme in two conformational states |
| 7.4.2.8 | cryo-electron microscopy, enzyme dodecamer comprises two hexameric rings that are stacked face-to-face by the association of their C-terminal regions |
| 7.4.2.8 | crystals of selenomethionine-substituted afGspE are grown in the presence of 10 mM AMP-PNP and 10 mM Mg2+, a resolution of 2.95 A is attained |
| 7.4.2.8 | FliHC2-FliI complex, sitting drop vapor diffusion method, using 0.1 M HEPES-NaOH (pH 7.2), 5% (w/v) PEG-400, and 0.1 M magnesium acetate |
| 7.4.2.8 | major cytoplasmic domain of EpsL protein that is part of the ATPase complex |
| 7.4.2.8 | N-terminal FHA domains (EssC-N) and a C-terminal fragment EssC-C, sitting drop vapor diffusion method, using 0.2 M magnesium formate and 20% (w/v) PEG3350 |
| 7.4.2.8 | PDB ID 2DPY |
| 7.4.2.8 | periplasmic domain of EpsM protein, fold is a circular permutation of the ferredoxin fold |
| 7.4.2.8 | purified recombinant enzyme GspE in complex with cyto-GspL, sitting drop vapor diffusion method, by mixing of 0.001 ml of protein in 20 mM HEPES, pH7.5, 200 mM NaCl buffer with 0.001 ml of reservoir solution containing 0.2 M Na malonate pH 7.0, 18% PEG 3350, at 21°C, X-ray diffraction structure determination and analysis at 2.83 A resolution, molecular replacement using the structures of Vibrio cholerae DELTAN1EGspE (PDB ID 1P9R) and the Vibrio cholerae N1E-cyto-GspL complex (PDB ID 2BH1) as search models |
