EC Number   |
|---|
   6.5.1.2 | - |
| 6.5.1.2 | adenylation domain of NAD+-dependent ligase with bound AMP |
| 6.5.1.2 | crystals of adenylation domain are grown by vapor diffusion using hanging drops. Crystals belong to space group C2 with unit cell parameters of a = 90.2 A, b = 86.1 A, c = 57.1 A and beta = 101°. Visualization of NAD+ recognition by an NAD+-dependent ligase |
| 6.5.1.2 | hanging drop vapor diffusion method, using 2.1-2.5 M ammonium sulfate and 0.1 M MES or HEPES pH 6.8-7.5 |
| 6.5.1.2 | hanging drop vapour phase diffusion method, K114A mutant protein fragment |
| 6.5.1.2 | microseeding technique |
| 6.5.1.2 | sitting drop vapor diffusion method at 22°C. 2.3 A crystal structure of Escherichia coli LigA bound to an adenylylated nick, which captures LigA in a state poised for strand closure and reveals the basis for nick recognition. LigA envelops the DNA within a protein clamp |
| 6.5.1.2 | vapor diffusion method |
