6.3.4.3 | native protein, folate complex, ADP-formylphosphate complex, and ZD9331-formylphosphate complex, hanging drop vapor diffusion method. High salt conditions contain 38-46% (w/v) saturated ammonium sulfate, 1 mM , and dithiohtreitol, 1-3.5% (w/v) PEG 1000 or PEG 1450, in 50-75 mM KMB (pH 7.0-8.0). Low salt conditions contain 18-22% (w/v) PEG 6-8K, 0.2 M ammonium sulfate, 1 mM dithiothreitol, in 75 mM KMB pH 7.0-8.5 |