EC Number |
---|
5.6.1.3 | - |
5.6.1.3 | catalytic domain in the ADP- and adenosine-5'-(beta,gamma-imido)triphosphate-bound states, hanging drop vapour diffusion method, using 0.1 MES (pH 6.5), 15% polyethylene glycol 20000, at 4°C |
5.6.1.3 | crystal structure analysis of ubiquitous kinesin motor domain and kinesin motor domain with docked neck linker, PDB IDs 1BG2 and 1MKJ, respectively |
5.6.1.3 | crystal structure analysis, PDB IDs 4HNA, 1BG2, and 1II6, structure modeling |
5.6.1.3 | His7-tagged KIF4 motor domain construct KIF4-344 in complex with AMPPNP, sitting drop vapour diffusion method, mixing of 15 mg/ml protein in 10 mM TrisHCl, pH 8.0, 100 mM NaCl, and 1 mM DTT with a reservoir solution containing 28% w/v PEG 4000, 100 mM Tris-HCl, pH 8.5, and 200 mM sodium acetate, in the presence of 5 mM AMPPNP, X-ray diffraction strcuture determination and analysis at 1.71 A resolution, molecular replacement and modeling |
5.6.1.3 | KIF1A-ADP crystals are grown by vapour diffusion, 0.002 ml protein solution containing approx. 15 mg/ml protein are mixed with 0.002 ml reservoir buffer consisting of 30% polyethylene glycol 4000, 100 mM Tris-HCl, pH 8.5, 200 mM sodium acetate, 8% sucrose, 4 mM ADP and 10 mM MgCl2, crystals of KIF1A-beta,gamma-methyleneadenosine 5-triphosphate are obtained using 27% polyethylene glycol 4000, 100 mM MES-NaOH, pH 6.5 and 200 mM sodium acetate as reservoir buffer, crystals are soaked in buffer containing 20 mM beta,gamma-methyleneadenosine 5-triphosphate and 40 mM MgCl2 before data collection, crystals of KIF1A and KIF1A-beta,gamma-methyleneadenosine 5-triphosphate diffract to 2.2 and 2.0 A, respectively |
5.6.1.3 | purified recombinant Eg5 motor domain, residues 1-369, in complex with inhibitor PVZB1194, sitting drop vapor diffusion method, at 4°C, mixing of 500 nl of 6 mg/ml protein in 50 mM PIPES-NaOH, pH 6.8, 250 mM NaCl, 2 mM MgCl2, 1 mM EGTA-NaOH, 1 mM TCEP-HCl, and 10% w/v sucrose, and inhibitor PVZB1194 (ratio 1:3 or 1:4 with enzyme) solution with 500 nl of the reservoir solution containing 19% w/v PEG 3350, 0.1 M MES-NaOH, pH 6.0, and 200 mM NaNO3, 1 week, followed by microseeding, X-ray diffraction structure determination and analysis at 2.8 A resolution |
5.6.1.3 | purified recombinant minimal functional domain Kif2C-(sN+M)-sL2 in complex with ADP, by vapor diffusion using a crystallization buffer consisting of 0.1 M Tris-HCl, pH 8.0, 1.5 M ammonium sulfate, and 10% ethylene glycol, at 20°C, X-ray diffraction structure determination and analysis at 2.6 A resolution, molecular replacement using HsKif2C motor domain-ADP, PDB ID 2HEH, as a starting model |
5.6.1.3 | study of the discovery and optimization of hexahydr-2H-pyranol[3,2-c]quinolines, HHPQs as inhibitors. Crystallographic data demonstrate that these potent and selectve inhibitors bind in an allosteric pocket of kinesin-5 distant from the nucleotide and microtubule binding sites |
5.6.1.3 | truncated protein comprised of the conserved motor domain of NcKin3, the following 14 amino acids, and a Cys-tag, in complex with MgADP bound to the active site. The NcKin3 motor decorates microtubules at a stoichiometry of one head per alphabeta-tubulin heterodimer, forming an axial periodicity of 8 nm |