EC Number |
---|
5.3.1.5 | - |
5.3.1.5 | 3.0 A crystal structure |
5.3.1.5 | ammonium sulfate as precipitant, orthorhombic space group P212121 with a: 84.35 A, b: 123.6 A, c: 140.24 A |
5.3.1.5 | analysis of the location of hydrogen atoms by time-of-flight neutron Laue technique. The neutron structure of crystalline XI with bound product, D-xylulose, shows, that O5 of D-xylulose is not protonated but is hydrogen-bonded to doubly protonated His54. Also, Lys289, which is neutral in native XI, is protonated, while the catalytic water in native XI has become activated to a hydroxyl anion which is in the proximity of C1 and C2, the molecular site of isomerization of xylose |
5.3.1.5 | at room temperature with polyethylene glycol 4000 as precipitant, orthorhombic space group P212121 with a: 73.34 A, b: 144.05 A and c 155.07 A |
5.3.1.5 | cooling crystallization from 0.17 M MgSO4 solution |
5.3.1.5 | crystal structure of complexes of D-xylose isomerase with deoxysugars |
5.3.1.5 | D-threonohydroxamic acid soaked into the crystal, crystallographic structure at 1.6 A resolution |
5.3.1.5 | in complex with D-glyceraldehyde, hanging drop vapor diffusion method, using 0.2-0.3 M Mg-formate at pH 7.0 and 22°C |
5.3.1.5 | neutron diffraction, largest crystals at 18°C, 95 mg/ml xylose isomerase, 16.9% ammonium sulfate, mathematical analysis to determine optimal conditions for crystallization |