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EC Number	Crystallization (Commentary)
5.1.3.11	apoenzyme crystal structure comparisons and molecular replacement method using the structure of Ruminococcus albus enzyme (PDB ID 3WKF) as a search model, overview
5.1.3.11	purified recombinant enzyme in apoform and complexed with D-glucose-D-mannose, epilactose, and reaction intermediate analogue cellobiitol, sitting drop vapor diffusion method, mixing of 500 nl of 5 mg/ml protein in 30 mM Tris-HCl, pH 8.5, and 60 mM NaCl, with 500 nl reservoir solution containing 0.1 M sodium acetate, pH 4.5, and 1.2 M ammonium hydrogen phosphate, 5 days, 20°C, method optimization, X-ray diffraction structure determination and analysis at 1.74-2.19 A resolution

EC Number

Crystallization (Commentary)

5.1.3.11

apoenzyme crystal structure comparisons and molecular replacement method using the structure of Ruminococcus albus enzyme (PDB ID 3WKF) as a search model, overview

5.1.3.11

purified recombinant enzyme in apoform and complexed with D-glucose-D-mannose, epilactose, and reaction intermediate analogue cellobiitol, sitting drop vapor diffusion method, mixing of 500 nl of 5 mg/ml protein in 30 mM Tris-HCl, pH 8.5, and 60 mM NaCl, with 500 nl reservoir solution containing 0.1 M sodium acetate, pH 4.5, and 1.2 M ammonium hydrogen phosphate, 5 days, 20°C, method optimization, X-ray diffraction structure determination and analysis at 1.74-2.19 A resolution

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Release 2023.1 (January 2023)