EC Number   |
|---|
   4.2.99.18 | combination of dialysis and seeding techniques, sitting drop vapor diffusion method |
| 4.2.99.18 | crystal structure of the enzyme and that of its complex with 8-oxo-guanosine at 1.0 and 1.7 A resolution, respectively, are grown by sitting drops method |
| 4.2.99.18 | Crystals are grown by vapor diffusion. The 1.10-A resolution DNA-free and the 2.45-A resolution DNA-substrate complex structures capture substrate stabilization by Arg37 and reveal a distorted Zn3-ligand arrangement that reverts, after catalysis, to an ideal geometry suitable to hold rather than release cleaved DNA product. Coordinates and structure factors for the three structures (E261Q-phosphate, E261Q-AP DNA and Y72A-AP DNA) have been deposited with accession codes 2NQH, 2NQJ and 2NQ9 |
| 4.2.99.18 | crystals are produced in hanging drops, the structure is solved at a resolution of 2.5 A |
| 4.2.99.18 | crystals of zApe (74 mg/ml in 10 mM HEPES, pH 7.5 buffer) are grown by hanging drop vapor diffusion at 20°C using 200 mM ammonium acetate, 100 mM Bis-Tris pH 5.5, 7% PEG 3350, 2% glycerol and 0.8 mM lead acetate as the precipitating solution. The structure is solved at a resolution of 49.15-2.3 A |
| 4.2.99.18 | hanging-drop, vapor-diffusion method at 12°C. A single crystal of the mutant enzyme K129Q in complex with a 15-mer duplex DNA oligonucleotide containing 8-oxoG paired with C is used to collect a 2.7 A data |
| 4.2.99.18 | molecular modeling of inhibitors to crystal structure, PDB entry 1DE8 |
| 4.2.99.18 | molecular modeling of structure, based on the homology to the human enzyme |
| 4.2.99.18 | mutant C65A hApe1 protein is diluted to 10 mg/ml in 10 mM HEPES pH 7.5 and then crystallized by hanging drop vapor diffusion using 10 mM MES pH 6.0, 7.5 mM Sm(OAc)3, 4% dioxane, 10-20% PEG 8000 as the precipitating solution at 20°C. The structure is solved at a resolution of 33.0-1.9 A |
| 4.2.99.18 | purified enzyme, sitting drop vapor diffusion method, a single crystal is obtained from 0.1 M bicine, pH 9.0, with 20% PEG 6000, at 16°C, X-ray diffraction structure determination and analysis, molecular replacement using the structure of Bacillus subtilis AP endonuclease ExoA (PDB ID 5CFE) as template |
