EC Number   |
|---|
    3.5.1.23 | crystal structure of neutral CDase for the ceramide-free and C2-ceramide-bound forms |
| 3.5.1.23 | crystallization of recombinant truncated enzyme, X-ray diffraction structure determination and analysis at 2.6 A resolution |
| 3.5.1.23 | purified recombinant ADIPOR2 complexed with a free fatty acid molecule, X-ray diffraction structure determination and analysis |
| 3.5.1.23 | purified recombinant His6-tagged extracellular domain of nCDase, residues 99-780, in complex with phosphate, hanging drop vapor diffusion, mixing of equal volumes of 3 mg/ml protein in 100 mM NaCl, 10 mM HEPES, pH 7.0, with reservoir solution containing 0.2 M Li2SO4, 10% PEG 1000, and 0.1 M citrate-phosphate, pH 4.6, at 16°C, X-ray diffraction structure determination and analysis at 2.6 A resolution, molecular replacement using Pseudomonas aeruginosa bCDase structure, PDB ID 2ZWS, as template |
| 3.5.1.23 | purified recombinant N-glycosylated extracellular region of nCDase in complex with phosphate, X-ray diffraction structure determination and analysis at 2.6 A resolution |
| 3.5.1.23 | purified recombinant wild-type and mutant BRIL-fusion ACER3 enzyme reconstituted into 10:1 monoolein:cholesterol at a ratio of 1:1.5 protein:lipid by weight, from precipitant solution consisting of 34-40% PEG 400, 0.1 M HEPES, pH 7.5, 75 mM magnesium sulfate, and 5% DMSO, 5 days, X-ray diffraction structure determination and analysis at 2.70-2.85 A resolution |
| 3.5.1.23 | the crystal structures of neutral CDase from Pseudomonas aeruginosa in the C2-ceramide-bound and -unbound forms are determined at 2.2 and 1.4 A resolutions, respectively |
