EC Number   |
|---|
   3.4.24.27 | - |
| 3.4.24.27 | analysis of the crystal structure of enzyme complexed with substrates L-phenylalanine methyl ester and carbobenzoxy-L-aspartic, or with inhibitor carbobenzoxy-L-phenylalanine-phosphonamidate-L-leucyl-L-alanine, X-ray diffraction structure determination and analysis, modeling |
| 3.4.24.27 | commercial preparation of crystalline enzyme |
| 3.4.24.27 | crystal structure determination |
| 3.4.24.27 | crystallization of tetragonal thermolysin, at room temperature, structure determination and anaylsis, fluctuations and solvent-accessible surface areas, biological nanopores and water densities, and radial distributions of water and ions, overview |
| 3.4.24.27 | data in presence of 4 M NaCl, introduced into crystals originally grown without NaCl |
| 3.4.24.27 | multiple-solvent crystal structure determination cell dimensions dependent on isopropanol concentration |
| 3.4.24.27 | native thermolysin in complex with 3-methylaspirin, 3-methylaspirin ethyl ester, and 3-methylaspirin cyclopropyl ester, sitting drop vapor diffusion method, using water as reservoir solution, at 18°C |
| 3.4.24.27 | recrystallized 3 times from DMSO |
| 3.4.24.27 | resiudes H142, H146, and E166 coordinate the catalytic zinc, while E143 and H231 are required for the catalytic activity |
