EC Number   |
|---|
    3.2.1.8 | - |
| 3.2.1.8 | crystal structure of xylanase 10B in the native state and in complex with xylobiose |
| 3.2.1.8 | enzyme bound to ferulic acid-1,5-arabinofuranose-alpha-1,3-xylotriose, hanging drop vapour diffusion method, with 0.1 M cadmium chloride, 0.1 M sodium acetate (pH 4.6) and 30% (w/v) polyethylene glycol 400 |
| 3.2.1.8 | homology modeling of structure |
| 3.2.1.8 | homology modeling of structure. Residues Glu145 and Glu252 are the catalytic nucleophile and proton donor, respectively |
| 3.2.1.8 | in 0.1 M NaCl, 0.2 M MgCl2, 0.1 M Tris-HCl (pH 8.5), and 15% PEG 8000 |
| 3.2.1.8 | in complex with xylotriose and xylobiose |
| 3.2.1.8 | molecular modeling of structure |
| 3.2.1.8 | mutant S35C/N44H/Y61M/T62C/N63L/D65P/N66G/T101P/S102N, to 2.0 A resolution. The formation of disulfide bond S35C/T62C induces new C-C contacts and a conformational change in the N-terminus. The induced conformational change is key for stabilizing the N-terminal region and for constructing thermostable mutants without compromising the activity |
| 3.2.1.8 | mutants D144A, E78Q, in complex with substrate xylopentaose and product xylotriose |
