EC Number   |
|---|
   3.1.3.16 | - |
| 3.1.3.16 | coexpression of the human regulatory subunit and the catalytic subunit from Neurospora crassa in cultured insect cells results in formation of large crystals in the cytoplasm |
| 3.1.3.16 | hanging drop vapour diffusion method with 0.2 M MgCl2, 4.5% PEG 10000 (w/v) and 0.1 M HEPES (pH 7.5) |
| 3.1.3.16 | hanging drop vapour diffusion method, using 7-10% (w/v) PEG35000 and 0.1-0.15 M sodium citrate (pH 5.5) |
| 3.1.3.16 | in complex with inhibitor-2 using the sitting drop vapour diffusion method with 100 mM Tris-HCl, pH 7.5, 300 mM potassium acetate, 12% (w/v) polyethylene glycol 3350, or with 100 mM Tris-HCl, pH 8.6, 150 mM sodium citrate, and 20% (w/v) polyethylene glycol 3350 |
| 3.1.3.16 | in complex with nodularin-R and tautomycin, PP1:nodularin-R is crystallized in 20% (w/v) PEG 3350, 0.2 M NaI. PP1:tautomycin is crystallized in 0.1 M Tris pH 8.0, 30% (w/v) PEG 6K, 1M LiCl |
| 3.1.3.16 | phosphatase KAP |
| 3.1.3.16 | purified recombinant detagged apo-form of the catalytic subunit, apo-PPZ1cat, or fused to microcystin-LR (the marine toxin microcystin-LR is a potent cyclic peptide inhibitor of PP1), by hanging drop vapor diffusion method, mixing of enzyme in 20 mM Tris, pH 8.0, 500 mM NaCl, 0.5 mM Tris(2-carboxyethyl) phosphine (TCEP), and 1 mM MnCl21.8 M with ammonium citrate tribasic, pH 7.0, in a 1:1 ratio, and subsequent mixture in a 2:1 protein/crystallization condition ratio with reservoir solution containing 0.06 M citric acid, pH 4.1, and 16% w/v PEG 3350, X-ray diffraction structure determination and analysis at 2.4-2.6 A resolution, molecular replacement using PP1 stucture, PDB ID 4MOV, as the search model |
| 3.1.3.16 | purified recombinant enzyme bound to the regulatory 34 kDa myosin phosphatase targeting subunit MYPT1 residues 1-299, 8 mg/ml protein in 10 mM Tris, pH 7.5, 20 mM NaCl, 4 mM DTT, and 1 mM MnCl2, 20°C, hanging drop vapour diffusion method, mixed with an equal volume of reservoir solution containing 9.2% w/v PEG 5000 monomethylether, 35% v/v glycerol, and 200 mM NH4Cl, X-ray diffraction structure determination and analysis at 2.7 A resolution |
| 3.1.3.16 | purified recombinant enzyme Tk-PTP(form I) Tk-PTP(form II) Tk-PTP(G95A), sitting-drop vapor diffusion method, for enzyme form I: mixing 0.001 ml of 10 mg/ml protein solution with 0.001 ml of precipitant solution containing 0.1 M sodium citrate, pH 5.4, 8% w/v PEG 10000, and 14% v/v dioxane, for enzyme form II: mixing of mixing 0.001 ml of 10 mg/ml protein solution with 0.001 ml of precipitant solution containing 0.03 M citric acid, 0.07 M Bis-Tris propane, pH 7.6, 8% w/v PEG 3350, and 0.08 M spermine tetrahydrochloride, and for mutant Tk-PTP(G95A): mixing of 0.001 ml of 10 mg/ml protein solution with 0.001 ml of precipitant solution containing 0.1 M Bis-Tris, pH 6.25, and 0.8 M magnesium formate dehydrate, all at 18°C, X-ray diffraction structure determination and analysis at 1.7-2.3 A resolution, molecular replacement method, model building |
