EC Number   |
|---|
   3.1.13.2 | - |
| 3.1.13.2 | crystal structures of full-length HIV-1 reverse transcriptase with a DNA/DNA or RNA/DNA substrate show a distance of about 60 A between the polymerase active site and the RNase H active site |
| 3.1.13.2 | enzyme bound to inhibitor (E)-3,4-dihydroxy-N'-((2-methoxynaphthalen-1-yl)methylene)benzohydrazide, by vapor diffusion in microseeded hanging drops containing equal volumes of protein and reservoir solution containing 50 mM imidazole, pH 6.4, 100 mM ammonium sulfate, 15 mM magnesium sulfate, 5% glucose, 11.5% PEG 8000 at 4°C, mixing 0.0074 ml of 20 mM inhibitor in DMSO with 0.0025 ml of 20% beta-octyl glucopyranoside, of this solution, 0.0075 ml is combined with 0.065 ml of 40 mg/ml RT and 0.0575 ml of additional RT buffer containing 10 mM Tris, pH 8.0, and 75 mM NaCl on ice, X-ray diffraction structure determination and anaylsis at 3.15 A resolution |
| 3.1.13.2 | hanging drop vapor diffusion method, using 40% (w/v) PEG1500, 100 mM Na citrate pH 4.7, and 5 mM MgCl2 |
| 3.1.13.2 | in complex with selenium-modified DNA/RNA, sitting drop vapor diffusion method, using 0.1 M MES pH 6.5, 12% (w/v) PEG 20000 |
| 3.1.13.2 | more: solution of structure of enzyme is determined by NMR methods in presence of Mg2+ |
| 3.1.13.2 | purified recombinant mutant Mo-MLV RNase H lacking the putative helix C complexed with the DNA/RNA hybrid substrate, DELTAC monomers and dimers, equilibration against a reservoir solution of 30% PEG 4000 and 0.2 M ammonium sulfate, protein solution, containing 7-10 mg/ml, 10 mM HEPES, pH 7.0, 150 mM NaCl, 5 mM DTT, and 0.1 mM EDTA, is mixed with reservoir solution at pH 5.2-6.0, DELTAC monomer crystallization fails to yield single, large crystals, DELTAC monomer crystals are soaked in 15% PEG 4000, 20% PEG 400, 0.1 M ammonium sulfate, 150 mM NaCl, 1% 2-propanol, 1.25% PEG MME 550, 5 mM MES, pH 6.5, and 0.5 mM zinc sulfate for 5 min, X-ray diffractions tructure determination and analysis at 1.6 A resolution, modelling |
| 3.1.13.2 | sitting drop vapor diffusion method, using 0.1 M imidazole pH 8.0, 0.2 M NaCl, 0.4 M NaH2PO4, and 1.6 M K2HPO4 |
| 3.1.13.2 | sitting drop vapor diffusion method, using 27% (w/v) polyethylene glycol 1500 and 0.1 M sodium citrate, pH 4.7 |
