EC Number   |
|---|
    3.1.1.23 | apoenzyme alone or in complex with inhibitor SAR629, in 50 mM MES (pH 6.0) and 40% (v/v) 2-methyl-pentane-2,4-diol, at 4°C |
| 3.1.1.23 | by the hanging drop method and under oil crystallization, native enzyme or selenomethionyl derivative, at 2.2 A resolution. Belongs to I222 space group, with two molecules per asymmetric unit. Docking of 2-arachidonoylglycerol highlights a hydrophobic and a hydrophilic cavity that accommodate the lipid into the catalytic site |
| 3.1.1.23 | enzyme mtbMGL in open conformation, sitting drop vapor diffusion method and microseeding, mixing 500 nl of 10 mg/ml protein solution with 500 nl of crystallization solution containing 0.1 M carboxylic acids, 0.1 M sodium HEPES/MOPS, pH 7.5, 20% ethylene glycol, and 10% PEG 8000, 4 months, 20°C, microseeding by mixing of 400 nl protein solution with 400 nl crystallization solution and 200 nl seeding stock, containing 0.03 M NaNO3, 0.03 M Na2HPO4, 0.03 M (NH4)2SO4, 0.1 M Na HEPES/MOPS, pH 7.8, 12% MPD, 12% PEG 1000 and 12% PEG 3350, 2 weeks, 20°C, X-ray diffraction structure determination and analysis at 1.80 A resolution, molecular replacement using the PDB IDs 3PE6 and 1W53 as search templates |
| 3.1.1.23 | hanging drop vapor diffusion method, benzamidine is essential for crystallization |
| 3.1.1.23 | hanging drop vapor diffusion method, using 1.5 M ammonium sulfate, 100 mM citrate (pH 5.8) and 100 mM tartrate |
| 3.1.1.23 | in complex with methyl arachidonyl fluorophosphonate, hanging drop vapor diffusion method, using 6-10% (w/v) PEGMME 5000, 100 mM Na-MES pH 6.0, 0.2% (w/v) glucopyranoside, at 22°C |
| 3.1.1.23 | sitting drop vapor diffusion method, using 0.1 M MES/imidazole pH 6.5, 12.5% (w/v) PEG 1000, 12.5% (w/v) PEG 3350, 12.5% (w/v) MPD |
| 3.1.1.23 | sitting drop vapor diffusion method, using 0.1 M MES/imidazole, pH 6.5, 12.5% (w/v) PEG 1000, 12.5% (w/v) PEG 3350, 12.5% (w/v) 2-methyl-2,4-pentanediol or hanging drop vapor diffusion method using 0.1 M citric acid, pH 5.2, and 18% (w/v) PEG3350 |
