EC Number   |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
|---|
    3.2.1.23 | -999 |
- |
more |
turnover numbers of chimeric galactosidases |
171341 |
| 3.2.1.23 | 0.01 |
0.08 |
2-nitrophenyl-beta-D-galactopyranoside |
25°C |
682849 |
| 3.2.1.23 | 0.17 |
- |
2,4-dinitrophenyl 2-acetamido-2-deoxy-alpha-D-galactopyranosyl-(1-3)-[alpha-L-fucopyranosyl-(1-2)]-beta-D-galactopyranoside |
mutant enzyme E354A, in 20 mM sodium acetate buffer at pH 6.0, 37°C |
702342 |
| 3.2.1.23 | 0.21 |
- |
2-nitrophenyl-beta-D-galactopyranoside |
pH 6.5, 65°C, mutant E313G |
678474 |
| 3.2.1.23 | 0.32 |
- |
2-nitrophenyl-beta-D-galactopyranoside |
pH 6.5, 65°C, mutant E157G |
678474 |
| 3.2.1.23 | 1 |
- |
o-nitrophenyl-beta-D-galactoside |
isoenzyme betaG41 |
171290 |
| 3.2.1.23 | 1.2 |
- |
lactose |
- |
655727 |
| 3.2.1.23 | 1.35 |
- |
2,4-dinitrophenyl 2-acetamido-2-deoxy-alpha-D-galactopyranosyl-(1-3)-[alpha-L-fucopyranosyl-(1-2)]-beta-D-galactopyranoside |
mutant enzyme D429A, in 20 mM sodium acetate buffer at pH 6.0, 37°C |
702342 |
| 3.2.1.23 | 1.55 |
- |
2,4-dinitrophenyl 2-acetamido-2-deoxy-alpha-D-galactopyranosyl-(1-3)-[alpha-L-fucopyranosyl-(1-2)]-beta-D-galactopyranoside |
mutant enzyme E467A, in 20 mM sodium acetate buffer at pH 6.0, 37°C |
702342 |
| 3.2.1.23 | 1.7 |
- |
4-methylumbelliferyl beta-D-galactoside |
- |
655727 |
