EC Number   |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
|---|
    3.2.1.21 | -999 |
- |
more |
in the presence of 50 mM azide, the apparent kcat values of the E176Q and E176A mutants are nearly independent of the pH from pH 5-10, while those of the wild-type and E176D enzymes decrease 30 and 8fold, respectively, as the pH increases from 5 to 10 |
716056 |
| 3.2.1.21 | 0.000285 |
- |
4-nitrophenyl-beta-D-galactoside |
mutant enzyme E451A, at 30°C |
702975 |
| 3.2.1.21 | 0.00038 |
- |
4-nitrophenyl-beta-D-galactoside |
mutant enzyme Q39N, at 30°C |
702975 |
| 3.2.1.21 | 0.0004 |
- |
4-nitrophenyl-beta-D-fucoside |
mutant enzyme Q39N/E451S, at 30°C |
702975 |
| 3.2.1.21 | 0.00053 |
- |
4-nitrophenyl-beta-D-fucoside |
mutant enzyme E451S, at 30°C |
702975 |
| 3.2.1.21 | 0.0006 |
- |
4-nitrophenyl-beta-D-galactoside |
mutant enzyme Q39N/E451S, at 30°C |
702975 |
| 3.2.1.21 | 0.00061 |
- |
4-nitrophenyl-beta-D-fucoside |
mutant enzyme E451A, at 30°C |
702975 |
| 3.2.1.21 | 0.00093 |
- |
4-nitrophenyl-beta-D-galactoside |
mutant enzyme E451S, at 30°C |
702975 |
| 3.2.1.21 | 0.001 |
- |
cellobiose |
mutant enzyme E490A, in 0.1 M sodium acetate, pH 4.0, at 40°C |
729287 |
| 3.2.1.21 | 0.00188 |
- |
4-nitrophenyl beta-D-xyloside |
mutant E190A, 30°C, pH 6.0 |
697866 |
