EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
3.1.3.16 | 0.0015 |
- |
4-nitrophenyl phosphate |
mutant enzyme D136Y, with 0.05 mM Mn2+, in 50 mM Tris-HCl (pH 8.0), at 37°C |
730314 |
3.1.3.16 | 0.0038 |
- |
4-nitrophenyl phosphate |
mutant enzyme S120R, with 0.05 mM Mn2+, in 50 mM Tris-HCl (pH 8.0), at 37°C |
730314 |
3.1.3.16 | 0.014 |
- |
4-nitrophenyl phosphate |
mutant enzyme T101R, with 2 mM Mn2+, in 50 mM Tris-HCl (pH 8.0), at 37°C |
730314 |
3.1.3.16 | 0.0143 |
- |
6,8-difluoro-4-methylumbelliferyl phosphate |
pH 5.0, 20°C, mutant D63N |
751975 |
3.1.3.16 | 0.03 |
- |
RGRGSpSVGGGS |
at pH 7.5 and, temperature not specified in the publication |
748177 |
3.1.3.16 | 0.0371 |
- |
6,8-difluoro-4-methylumbelliferyl phosphate |
pH 5.0, 20°C, mutant R124E |
751975 |
3.1.3.16 | 0.0415 |
- |
6,8-difluoro-4-methylumbelliferyl phosphate |
pH 5.0, 20°C, mutant D63A |
751975 |
3.1.3.16 | 0.0652 |
- |
6,8-difluoro-4-methylumbelliferyl phosphate |
pH 5.0, 20°C, mutant D63N/E132A |
751975 |
3.1.3.16 | 0.09 |
- |
RRA(pT)VA |
pH 9.0, 30°C, recombinant wild-type enzyme |
751129 |
3.1.3.16 | 0.1 |
- |
p-nitrophenyl phosphate |
pH 7.8, H186N |
654611 |