EC Number   |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
|---|
    3.1.2.20 | -999 |
- |
more |
D44A mutant protein: value below 0.001 s-1 for isobutyryl-CoA and lauroyl-CoA |
707475 |
| 3.1.2.20 | -999 |
- |
more |
inactive with acetyl-CoA and 2-butenoyl-CoA |
707430 |
| 3.1.2.20 | -999 |
- |
more |
inactive with substrates having acyl chains with fewer than 12 carbon atoms |
707477 |
| 3.1.2.20 | -999 |
- |
more |
no activity observed with 4-hydroxybenzoyl-CoA, lauroyl-acyl carrier protein, myristoyl-acyl carrier protein, palmitoyl-acyl carrier protein, octanoyl-acyl carrier protein, oleoyl-acyl carrier protein and acylated peptide N-acetyl-Gly-Ala-Cys-S-palmitoyl, turnover number of mutant protein D65A below 0.00001 s-1 for 3-hydroxyphenylacetyl-CoA |
707486 |
| 3.1.2.20 | -999 |
- |
propionyl-S-n-acetylcysteamine |
absorbance of 5-thio-2-nitrobenzoate formed by reaction of 5,5'-dithiobis(2-nitrobenzoic acid) is measured, Kcat is above 0.0001 |
690932 |
| 3.1.2.20 | 0.0009 |
- |
palmitoyl-CoA |
at pH 7.5 and 30°C |
749670 |
| 3.1.2.20 | 0.00217 |
- |
2,3-dihydroxybenzoyl-CoA |
mutant H54A, at room temperature |
696310 |
| 3.1.2.20 | 0.0032 |
- |
4-chlorobenzoyl-CoA |
wild-type protein, pH7.5, 25°C |
707486 |
| 3.1.2.20 | 0.00333 |
- |
2,3-dihydroxybenzoyl-CoA |
mutant S67C, at room temperature |
696310 |
| 3.1.2.20 | 0.0044 |
- |
palmitoyl-CoA |
wild-type protein, pH7.5, 25°C |
707486 |
